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Open AccessArticle

New Molecular Tool for a Quick and Easy Detection of Apple Scab in the Field

1
Centre of Competence for the Innovation in the Agro-environmental Sector—AGROINNOVA, University of Turin, via Paolo Braccini 2, I-10095 Grugliasco, Italy
2
Department of Agricultural, Forestry and Food Sciences (DiSAFA), University of Torino, via Paolo Braccini 2, I-10095 Grugliasco, Italy
*
Author to whom correspondence should be addressed.
Agronomy 2020, 10(4), 581; https://doi.org/10.3390/agronomy10040581
Received: 30 March 2020 / Revised: 15 April 2020 / Accepted: 15 April 2020 / Published: 18 April 2020
Venturia inaequalis, an agent of apple scab, is the most important pathogen of Malus x domestica. Control measures against this pathogen rely on intensive phytosanitary programs based on predictive models to identify the meteorological conditions conducive to the primary infection. The detection of the pathogen in field, both in naturally infected symptomatic and asymptomatic leaves, is desirable. Loop-mediated isothermal amplification (LAMP) assays are profitable molecular diagnostic tools for the direct detection of pathogens in field. A LAMP assay for V. inaequalis has been designed on the elongation factor 1-alpha sequence. The validation of the LAMP assay was carried out following the international EPPO standard PM 7/98 in terms of specificity, sensitivity, repeatability and reproducibility. Specificity testing was performed using target and non-target species, such as phylogenetically related Venturia species and other pathogens commonly found in apple, resulting in positive amplification only for the target with a time to positive ranging from 20 to 30 min. Sensitivity testing was performed with serial dilutions of DNA of the target and by artificial inoculation of young apple leaves. The reliability of the LAMP assay as an early-detection tool and its user-friendly application make it suitable for the diagnosis of apple scab in the field. View Full-Text
Keywords: apple scab; diagnosis; on-site; LAMP apple scab; diagnosis; on-site; LAMP
MDPI and ACS Style

Franco Ortega, S.; Prencipe, S.; Gullino, M.L.; Spadaro, D. New Molecular Tool for a Quick and Easy Detection of Apple Scab in the Field. Agronomy 2020, 10, 581.

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