Identification and Validation a Major QTL from “Sea Rice 86” Seedlings Conferred Salt Tolerance
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Phenotype Measurements
2.3. DNA-BSA Analysis
2.4. Genotype Identification and QTL Analysis
2.5. Measurements of Agronomic Traits
2.6. Statistical Analysis
3. Results
3.1. Phenotypes and Genetics Analysis of Salt Tolerance in SR86
3.2. A New Potential QTL (qST1.1) Involved in the Salt Tolerance of SR86
3.3. qST1.1 Validation by Substitution Experiments and Introgression Line T22
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Soil Conditions (8 d) | Hydroponic Conditions (4 d) | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Variety/NaCl Concentration (%) | 0 | 0.6 | 0.8 | 1 | 1.2 | 1.5 | 0 | 0.4 | 0.6 | 0.8 | 1 |
SR86 | 1 | 1–3 | 1–3 | 1–3 | 1–3 | 3–5 | 1 | 3–5 | 5–7 | 7–9 | 9 |
DJY1 | 1 | 1–3 | 5–7 | 7–9 | 9 | 9 | 1 | 3 | 5 | 7–9 | 9 |
YJ26 | 1 | 1–3 | 5–7 | 7–9 | 9 | 9 | 1 | 3 | 5 | 7–9 | 9 |
YJ29 | 1 | 1–3 | 5–7 | 7–9 | 9 | 9 | 1 | 3 | 5 | 7–9 | 9 |
YJ32 | 1 | 1–3 | 5–7 | 7–9 | 9 | 9 | 1 | 3 | 5 | 7–9 | 9 |
Chromosome ID | Start | End | Size (Mb) | Gene Number |
---|---|---|---|---|
Chr1 | 9,459,487 | 12,134,056 | 2.67 | 432 |
Chr1 | 12,211,515 | 12,244,801 | 0.03 | 5 |
Total | - | - | 2.70 | 437 |
Traits | SR86 | T22 | YJ26 |
---|---|---|---|
Plant height (cm) | 163.22 ± 6.10 | 100.52 ± 3.44 * | 95.56 ± 5.63 |
Number of filled grains per panicle | 71.07 ± 15.74 | 110.70 ± 8.90 ** | 83.33 ± 22.32 |
Seed setting rate | 0.74 ± 0.10 | 0.77 ± 0.04 ** | 0.86 ± 0.04 |
Effective tiller | 11.78 ± 2.33 | 10.11 ± 1.21 | 8.77 ± 2.33 |
1000-grain weight (g) | 25.59 ± 0.85 | 26.77 ± 0.72 ** | 28.44 ± 0.52 |
Panicle length (cm) | 22.68 ± 0.75 | 23.50 ± 0.90 * | 17.60 ± 0 .93 |
Primary branching | 9.82 ± 0.16 | 10.3 1± 0.63 * | 8.96 ± 0.54 |
Secondary branching | 27.00 ± 2.75 | 26.33 ± 3.05 ** | 18.05 ± 2.56 |
Grain length (mm) | 7.19 ± 0.24 | 7.81 ± 0.07 ** | 7.12 ± 0.05 |
Grain width (mm) | 2.76 ± 0.08 | 2.83 ± 0.36 ** | 3.07 ± 0.06 |
Grain length-to-width ratio | 2.64 ± 0.50 | 2.82 ± 0.03 ** | 2.36 ± 0.05 |
Grain yield per plant (g) | 22.21 ± 6.51 | 29.32 ± 3.03 ** | 19.88 ± 2.43 |
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Wu, F.; Yang, J.; Yu, D.; Xu, P. Identification and Validation a Major QTL from “Sea Rice 86” Seedlings Conferred Salt Tolerance. Agronomy 2020, 10, 410. https://doi.org/10.3390/agronomy10030410
Wu F, Yang J, Yu D, Xu P. Identification and Validation a Major QTL from “Sea Rice 86” Seedlings Conferred Salt Tolerance. Agronomy. 2020; 10(3):410. https://doi.org/10.3390/agronomy10030410
Chicago/Turabian StyleWu, Fengling, Jun Yang, Diqiu Yu, and Peng Xu. 2020. "Identification and Validation a Major QTL from “Sea Rice 86” Seedlings Conferred Salt Tolerance" Agronomy 10, no. 3: 410. https://doi.org/10.3390/agronomy10030410