Next Article in Journal
Synthesis and Engineering of Hyaluronic Acid-Gelatin Hydrogels with Improved Cellular Attachment and Growth
Next Article in Special Issue
Modification of Processability and Shear-Induced Crystallization of Poly(lactic acid)
Previous Article in Journal
Novel Treatment of 3D-Printed Short-Carbon-Fiber-Reinforced Polyamide (3D-SCFRPA66) Using Homogeneous Low-Potential Electron Beam Irradiation (HLEBI) and Ductility Enhancement
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Polymers
Volume 16
Issue 23
10.3390/polym16233407
polymers-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Producing and Characterizing Polyhydroxyalkanoates from Starch and Chickpea Waste Using Mixed Microbial Cultures in Solid-State Fermentation

by
Karlo Grgurević
1,
Dora Bramberger
1,
Martina Miloloža
1,
Krešimir Stublić
2,
Vesna Ocelić Bulatović
1,
Jasmina Ranilović
3,
Šime Ukić
1,
Tomislav Bolanča
1,
Matija Cvetnić
1,
Marinko Markić
1 and
Dajana Kučić Grgić
1,*
1
Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, Croatia
2
Aqua V.M.V. d.o.o., Kralja Zvonimira 98, 10000 Zagreb, Croatia
3
Podravka Inc., Ante Starčevića 32, 48000 Koprivnica, Croatia
*
Author to whom correspondence should be addressed.
Polymers 2024, 16(23), 3407; https://doi.org/10.3390/polym16233407
Submission received: 8 November 2024 / Revised: 29 November 2024 / Accepted: 2 December 2024 / Published: 3 December 2024
(This article belongs to the Special Issue Biodegradable Polymers: Innovations in Processing, Diverse Applications, and Sustainable Solutions)
Browse Figures
Versions Notes

Abstract

The environmental impact of plastic waste is a growing global challenge, primarily due to non-biodegradable plastics from fossil resources that accumulate in ecosystems. Biodegradable polymers like polyhydroxyalkanoates (PHAs) offer a sustainable alternative. PHAs are microbial biopolymers produced by microorganisms using renewable substrates, including agro-industrial byproducts, making them eco-friendly and cost-effective. This study focused on the isolation and characterization of PHA-producing microorganisms from agro-industrial waste, including chickpeas, chickpeas with bean residues, and starch. Screening via Sudan Black staining identified PHA-accumulating strains such as Brevibacillus sp., Micrococcus spp., and Candida krusei, among others. To assess the potential for PHA biosynthesis, solid-state fermentation (SSF) was conducted using agro-industrial waste as substrates, along with a mixed culture of the isolated microorganisms. The highest observed yield was a PHA accumulation of 13.81%, achieved with chickpeas containing bean residues. Structural and thermal characterization of the PHAs was performed using Fourier-transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). FTIR-ATR spectra indicated polyhydroxybutyrate (PHB), suggesting it as the synthesized PHA type. This study highlights the potential of agro-industrial waste for sustainable PHA production and eco-friendly bioplastics.
Keywords: agro-industrial waste; isolation and identification; solid-state fermentation; polyhydroxyalkanoates agro-industrial waste; isolation and identification; solid-state fermentation; polyhydroxyalkanoates

Share and Cite

MDPI and ACS Style

Grgurević, K.; Bramberger, D.; Miloloža, M.; Stublić, K.; Ocelić Bulatović, V.; Ranilović, J.; Ukić, Š.; Bolanča, T.; Cvetnić, M.; Markić, M.; et al. Producing and Characterizing Polyhydroxyalkanoates from Starch and Chickpea Waste Using Mixed Microbial Cultures in Solid-State Fermentation. Polymers 2024, 16, 3407. https://doi.org/10.3390/polym16233407

AMA Style

Grgurević K, Bramberger D, Miloloža M, Stublić K, Ocelić Bulatović V, Ranilović J, Ukić Š, Bolanča T, Cvetnić M, Markić M, et al. Producing and Characterizing Polyhydroxyalkanoates from Starch and Chickpea Waste Using Mixed Microbial Cultures in Solid-State Fermentation. Polymers. 2024; 16(23):3407. https://doi.org/10.3390/polym16233407

Chicago/Turabian Style

Grgurević, Karlo, Dora Bramberger, Martina Miloloža, Krešimir Stublić, Vesna Ocelić Bulatović, Jasmina Ranilović, Šime Ukić, Tomislav Bolanča, Matija Cvetnić, Marinko Markić, and et al. 2024. "Producing and Characterizing Polyhydroxyalkanoates from Starch and Chickpea Waste Using Mixed Microbial Cultures in Solid-State Fermentation" Polymers 16, no. 23: 3407. https://doi.org/10.3390/polym16233407

APA Style

Grgurević, K., Bramberger, D., Miloloža, M., Stublić, K., Ocelić Bulatović, V., Ranilović, J., Ukić, Š., Bolanča, T., Cvetnić, M., Markić, M., & Kučić Grgić, D. (2024). Producing and Characterizing Polyhydroxyalkanoates from Starch and Chickpea Waste Using Mixed Microbial Cultures in Solid-State Fermentation. Polymers, 16(23), 3407. https://doi.org/10.3390/polym16233407

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop