Muscle-like Scaffolds for Biomechanical Stimulation in a Custom-Built Bioreactor

Rojas-Rojas, Laura; Espinoza-Álvarez, María Laura; Castro-Piedra, Silvia; Ulloa-Fernández, Andrea; Vargas-Segura, Walter; Guillén-Girón, Teodolito

doi:10.3390/polym14245427

Open AccessArticle

Muscle-like Scaffolds for Biomechanical Stimulation in a Custom-Built Bioreactor

by

Laura Rojas-Rojas

^1,2,*

,

María Laura Espinoza-Álvarez

^1,3

,

Silvia Castro-Piedra

³

,

Andrea Ulloa-Fernández

³,

Walter Vargas-Segura

²

and

Teodolito Guillén-Girón

¹

Materials Science and Engineering School, Instituto Tecnológico de Costa Rica, Cartago 30101, Costa Rica

²

Physics School, Instituto Tecnológico de Costa Rica, Cartago 30101, Costa Rica

³

Biology School, Instituto Tecnológico de Costa Rica, Cartago 30101, Costa Rica

^*

Author to whom correspondence should be addressed.

Polymers 2022, 14(24), 5427; https://doi.org/10.3390/polym14245427

Submission received: 14 October 2022 / Revised: 21 November 2022 / Accepted: 23 November 2022 / Published: 11 December 2022

(This article belongs to the Special Issue Polymers for Biomedical Engineering and Applications)

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Abstract

Tissue engineering aims to develop in-vitro substitutes of native tissues. One approach of tissue engineering relies on using bioreactors combined with biomimetic scaffolds to produce study models or in-vitro substitutes. Bioreactors provide control over environmental parameters, place and hold a scaffold under desired characteristics, and apply mechanical stimulation to scaffolds. Polymers are often used for fabricating tissue-engineering scaffolds. In this study, polycaprolactone (PCL) collagen-coated microfilament scaffolds were cell-seeded with C2C12 myoblasts; then, these were grown inside a custom-built bioreactor. Cell attachment and proliferation on the scaffolds were investigated. A loading pattern was used for mechanical stimulation of the cell-seeded scaffolds. Results showed that the microfilaments provided a suitable scaffold for myoblast anchorage and that the custom-built bioreactor provided a qualified environment for the survival of the myoblasts on the polymeric scaffold. This PCL-based microfilament scaffold located inside the bioreactor proved to be a promising structure for the study of skeletal muscle models and can be used for mechanical stimulation studies in tissue engineering applications.

Keywords: tissue engineering; myoblasts; viability; cell adhesion; bioreactor

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MDPI and ACS Style

Rojas-Rojas, L.; Espinoza-Álvarez, M.L.; Castro-Piedra, S.; Ulloa-Fernández, A.; Vargas-Segura, W.; Guillén-Girón, T. Muscle-like Scaffolds for Biomechanical Stimulation in a Custom-Built Bioreactor. Polymers 2022, 14, 5427. https://doi.org/10.3390/polym14245427

AMA Style

Rojas-Rojas L, Espinoza-Álvarez ML, Castro-Piedra S, Ulloa-Fernández A, Vargas-Segura W, Guillén-Girón T. Muscle-like Scaffolds for Biomechanical Stimulation in a Custom-Built Bioreactor. Polymers. 2022; 14(24):5427. https://doi.org/10.3390/polym14245427

Chicago/Turabian Style

Rojas-Rojas, Laura, María Laura Espinoza-Álvarez, Silvia Castro-Piedra, Andrea Ulloa-Fernández, Walter Vargas-Segura, and Teodolito Guillén-Girón. 2022. "Muscle-like Scaffolds for Biomechanical Stimulation in a Custom-Built Bioreactor" Polymers 14, no. 24: 5427. https://doi.org/10.3390/polym14245427

APA Style

Rojas-Rojas, L., Espinoza-Álvarez, M. L., Castro-Piedra, S., Ulloa-Fernández, A., Vargas-Segura, W., & Guillén-Girón, T. (2022). Muscle-like Scaffolds for Biomechanical Stimulation in a Custom-Built Bioreactor. Polymers, 14(24), 5427. https://doi.org/10.3390/polym14245427

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Muscle-like Scaffolds for Biomechanical Stimulation in a Custom-Built Bioreactor

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