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Open AccessArticle

Topography-Mediated Myotube and Endothelial Alignment, Differentiation, and Extracellular Matrix Organization for Skeletal Muscle Engineering

1
Department of Pathology and Medical Biology, University Medical Center Groningen, Hanzeplein 1 (EA11), 9713 GZ Groningen, The Netherlands
2
Department of Biomedical Engineering-FB40, W.J. Kolff Institute for Biomedical Engineering and Materials Science-FB41, University Medical Center Groningen, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Polymers 2020, 12(9), 1948; https://doi.org/10.3390/polym12091948
Received: 13 July 2020 / Revised: 14 August 2020 / Accepted: 24 August 2020 / Published: 28 August 2020
Understanding the response of endothelial cells to aligned myotubes is important to create an appropriate environment for tissue-engineered vascularized skeletal muscle. Part of the native tissue environment is the extracellular matrix (ECM). The ECM is a supportive scaffold for cells and allows cellular processes such as proliferation, differentiation, and migration. Interstitial matrix and basal membrane both comprise proteinaceous and polysaccharide components for strength, architecture, and volume retention. Virtually all cells are anchored to their basal lamina. One of the physical factors that affects cell behavior is topography, which plays an important role on cell alignment. We tested the hypothesis that topography-driven aligned human myotubes promote and support vascular network formation as a prelude to in vitro engineered vascularized skeletal muscle. Therefore, we used a PDMS-based topography substrate to investigate the influence of pre-aligned myotubes on the network formation of microvascular endothelial cells. The aligned myotubes produced a network of collagen fibers and laminin. This network supported early stages of endothelial network formation. View Full-Text
Keywords: myoblasts; topography; vascularization; endothelial cells; skeletal muscle; extracellular matrix myoblasts; topography; vascularization; endothelial cells; skeletal muscle; extracellular matrix
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MDPI and ACS Style

Almonacid Suarez, A.M.; Brinker, M.G.L.; Brouwer, L.A.; van der Ham, I.; Harmsen, M.C.; van Rijn, P. Topography-Mediated Myotube and Endothelial Alignment, Differentiation, and Extracellular Matrix Organization for Skeletal Muscle Engineering. Polymers 2020, 12, 1948.

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