Next Article in Journal
Barrier Properties of GnP–PA-Extruded Films
Previous Article in Journal
Influence of Polyol/Crosslinker Blend Composition on Phase Separation and Thermo-Mechanical Properties of Polyurethane Thin Films
Previous Article in Special Issue
Effect of Fructose and Ascorbic Acid on the Performance of Cross-Linked Fish Gelatin Films
Open AccessArticle

Inclusion of Cross-Linked Elastin in Gelatin/PEG Hydrogels Favourably Influences Fibroblast Phenotype

1
School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore
2
The Inter-Departmental Program for Biotechnology, Technion-Israel Institute of Technology, Haifa 32000, Israel
3
Department of Chemical Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel
4
Subramanian Venkatraman, Materials Science and Engineering, National University of Singapore, Singapore 119077, Singapore
*
Authors to whom correspondence should be addressed.
Polymers 2020, 12(3), 670; https://doi.org/10.3390/polym12030670
Received: 21 February 2020 / Revised: 11 March 2020 / Accepted: 13 March 2020 / Published: 17 March 2020
(This article belongs to the Special Issue Functional Gelatin)
The capacity of a biomaterial to innately modulate cell behavior while meeting the mechanical property requirements of the implant is a much sought-after goal within bioengineering. Here we covalently incorporate soluble elastin into a gelatin–poly (ethylene glycol) (PEG) hydrogel for three-dimensional (3D) cell encapsulation to achieve these properties. The inclusion of elastin into a previously optimized gelatin–PEG hydrogel was then evaluated for effects on entrapped fibroblasts, with the aim to assess the hydrogel as an extracellular matrix (ECM)-mimicking 3D microenvironment for cellular guidance. Soluble elastin was incorporated both physically and covalently into novel gelatin/elastin hybrid PEG hydrogels with the aim to harness the cellular interactivity and mechanical tunability of both elastin and gelatin. This design allowed us to assess the benefits of elastin-containing hydrogels in guiding fibroblast activity for evaluation as a potential dermal replacement. It was found that a gelatin–PEG hydrogel with covalently conjugated elastin, supported neonatal fibroblast viability, promoted their proliferation from 7.3% to 13.5% and guided their behavior. The expression of collagen alpha-1(COL1A1) and elastin in gelatin/elastin hybrid gels increased 16-fold and 6-fold compared to control sample at day 9, respectively. Moreover, cells can be loaded into the hydrogel precursor solution, deposited, and the matrix cross-linked without affecting the incorporated cells adversely, thus enabling a potential injectable system for dermal wound healing. View Full-Text
Keywords: polyethylene glycol hydrogel; myofibroblast; gelatin; elastin; cell encapsulation; dermal substitu polyethylene glycol hydrogel; myofibroblast; gelatin; elastin; cell encapsulation; dermal substitu
Show Figures

Figure 1

MDPI and ACS Style

Cao, Y.; Lee, B.H.; Irvine, S.A.; Wong, Y.S.; Bianco Peled, H.; Venkatraman, S. Inclusion of Cross-Linked Elastin in Gelatin/PEG Hydrogels Favourably Influences Fibroblast Phenotype. Polymers 2020, 12, 670.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop