Bovine Decellularized Amniotic Membrane: Extracellular Matrix as Scaffold for Mammalian Skin
Abstract
:1. Introduction
2. Materials and Methods
2.1. Decellularization of the BAM
2.2. Determination of DNA Content
2.2.1. Extraction of DNA
2.2.2. DNA Quantification
2.3. Cell Culture
Cell Seeding on DM and 3-(4,5-d imethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) Assay
2.4. Histological Analysis with Hematoxylin-Eosin
2.5. Scanning Electron Microscopy (SEM)
2.6. FTIR-ATR Spectroscopy
2.7. Differential Scanning Calorimetry (DSC)
2.8. Statistic Analysis
3. Results and Discussion
3.1. Decellularization of BAM and DNA Content
3.2. MTT Assay
3.3. Histological Analysis
3.4. Scanning Electron Microscopy
3.5. FTIR-ATR Spectroscopy
3.6. Differential Scanning Calorimetry (DSC)
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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No. | Protocols |
---|---|
I | SDS 0.1% for 4 h NaOH 0.1 M for 1 h PAA + ascorbic acid 0.1 for 12 h Ethanol 70% for 1 h PBS for 2 h |
II | SDS 0.1% for 4 h NaOH 0.1 M for 1 h PAA 0.15% + EtOH for 12 h NaOH 0.1 M for 1 h PAA for 1 h Ethanol 70% for 1 h PBS for 2 h |
III | Tween 80 for 4 h NaOH 0.1 M for 1 h, PAA + ascorbic acid 0.1 for 12 h Ethanol 70% for 1 h PBS for 2 h |
IV | Tween 80 for 4 h NaOH 0.1 M for 1 h PAA 0.15% + EtOH for 12 h NaOH 0.1 M for 1 h PAA for 1 h Ethanol 70% for 1 h PBS for 2 h |
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Villamil Ballesteros, A.C.; Segura Puello, H.R.; Lopez-Garcia, J.A.; Bernal-Ballen, A.; Nieto Mosquera, D.L.; Muñoz Forero, D.M.; Segura Charry, J.S.; Neira Bejarano, Y.A. Bovine Decellularized Amniotic Membrane: Extracellular Matrix as Scaffold for Mammalian Skin. Polymers 2020, 12, 590. https://doi.org/10.3390/polym12030590
Villamil Ballesteros AC, Segura Puello HR, Lopez-Garcia JA, Bernal-Ballen A, Nieto Mosquera DL, Muñoz Forero DM, Segura Charry JS, Neira Bejarano YA. Bovine Decellularized Amniotic Membrane: Extracellular Matrix as Scaffold for Mammalian Skin. Polymers. 2020; 12(3):590. https://doi.org/10.3390/polym12030590
Chicago/Turabian StyleVillamil Ballesteros, Andrea Catalina, Hugo Ramiro Segura Puello, Jorge Andres Lopez-Garcia, Andres Bernal-Ballen, Diana Lorena Nieto Mosquera, Diana Milena Muñoz Forero, Juan Sebastián Segura Charry, and Yuli Alexandra Neira Bejarano. 2020. "Bovine Decellularized Amniotic Membrane: Extracellular Matrix as Scaffold for Mammalian Skin" Polymers 12, no. 3: 590. https://doi.org/10.3390/polym12030590