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Open AccessArticle

Synergistic Effects on Incorporation of β-Tricalcium Phosphate and Graphene Oxide Nanoparticles to Silk Fibroin/Soy Protein Isolate Scaffolds for Bone Tissue Engineering

1
Center of 3D Printing & Organ Manufacturing, School of Fundamental Sciences, China Medical University (CMU), No. 77 Puhe Road, Shenyang North New Area, Shenyang 110122, China
2
Department of Orthodontics, School of Stomatology, China Medical University, Shenyang 110122, China
3
College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China
4
Institute of Natural Sciences, Yonsei University, Seoul 120-749, Korea
5
Center of Organ Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China
*
Authors to whom correspondence should be addressed.
Polymers 2020, 12(1), 69; https://doi.org/10.3390/polym12010069
Received: 3 December 2019 / Revised: 16 December 2019 / Accepted: 16 December 2019 / Published: 2 January 2020
(This article belongs to the Special Issue Biodegradable Polymer Scaffolds for Tissue Engineering)
In bone tissue engineering, an ideal scaffold is required to have favorable physical, chemical (or physicochemical), and biological (or biochemical) properties to promote osteogenesis. Although silk fibroin (SF) and/or soy protein isolate (SPI) scaffolds have been widely used as an alternative to autologous and heterologous bone grafts, the poor mechanical property and insufficient osteoinductive capability has become an obstacle for their in vivo applications. Herein, β-tricalcium phosphate (β-TCP) and graphene oxide (GO) nanoparticles are incorporated into SF/SPI scaffolds simultaneously or individually. Physical and chemical properties of these composite scaffolds are evaluated using field emission scanning electron microscope (FESEM), X-ray diffraction (XRD) and attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR). Biocompatibility and osteogenesis of the composite scaffolds are evaluated using bone marrow mesenchymal stem cells (BMSCs). All the composite scaffolds have a complex porous structure with proper pore sizes and porosities. Physicochemical properties of the scaffolds can be significantly increased through the incorporation of β-TCP and GO nanoparticles. Alkaline phosphatase activity (ALP) and osteogenesis-related gene expression of the BMSCs are significantly enhanced in the presence of β-TCP and GO nanoparticles. Especially, β-TCP and GO nanoparticles have a synergistic effect on promoting osteogenesis. These results suggest that the β-TCP and GO enhanced SF/SPI scaffolds are promising candidates for bone tissue regeneration. View Full-Text
Keywords: graphene oxide; β-tricalcium phosphate; silk fibroin; soy protein isolate; scaffold; osteogenesis graphene oxide; β-tricalcium phosphate; silk fibroin; soy protein isolate; scaffold; osteogenesis
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MDPI and ACS Style

Liu, F.; Liu, C.; Zheng, B.; He, J.; Liu, J.; Chen, C.; Lee, I.-S.; Wang, X.; Liu, Y. Synergistic Effects on Incorporation of β-Tricalcium Phosphate and Graphene Oxide Nanoparticles to Silk Fibroin/Soy Protein Isolate Scaffolds for Bone Tissue Engineering. Polymers 2020, 12, 69.

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