Novel Nano-Liposome Formulation for Dry Eyes with Components Similar to the Preocular Tear Film
AbstractDry eye is commonly treated with artificial tears; however, developing artificial tears similar to natural tears is difficult due to the complex nature of tears. We characterized and evaluated a novel artificial tear formulation with components similar to the lipid and aqueous constituents of natural tears. Nano-liposomes, composed in part of phosphatidylcholine, were dispersed in an aqueous solution of bioadhesive sodium hyaluronate. Liposome size, zeta potential, and physicochemical properties of the fresh and stored (4 °C) liposomal formulation were analyzed. In vitro tolerance was tested using human corneal and conjunctival cell lines by exposures of 15 min to 4 h. The tolerance of the liposomal formulation was evaluated in animals (rabbits). The average liposome size was 186.3 ± 7.0 nm, and the zeta potential was negative. The osmolarity of the formulation was 198.6 ± 1.7 mOsm, with a surface tension of 36.5 ± 0.4 mN/m and viscosity of 3.05 ± 0.02 mPa·s. Viability values in the human corneal and conjunctival cell lines were always >80%, even after liposomal formulation storage for 8 weeks. Discomfort and clinical signs after instillation in rabbit eyes were absent. The new formulation, based on phosphatidylcholine-liposomes dispersed in sodium hyaluronate has suitable components and characteristics, including high in vitro cell viability and good in vivo tolerance, to serve as a tear substitute. View Full-Text
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Vicario-de-la-Torre, M.; Caballo-González, M.; Vico, E.; Morales-Fernández, L.; Arriola-Villalobos, P.; De las Heras, B.; Benítez-del-Castillo, J.M.; Guzmán, M.; Millar, T.; Herrero-Vanrell, R.; Molina-Martínez, I.T. Novel Nano-Liposome Formulation for Dry Eyes with Components Similar to the Preocular Tear Film. Polymers 2018, 10, 425.
Vicario-de-la-Torre M, Caballo-González M, Vico E, Morales-Fernández L, Arriola-Villalobos P, De las Heras B, Benítez-del-Castillo JM, Guzmán M, Millar T, Herrero-Vanrell R, Molina-Martínez IT. Novel Nano-Liposome Formulation for Dry Eyes with Components Similar to the Preocular Tear Film. Polymers. 2018; 10(4):425.Chicago/Turabian Style
Vicario-de-la-Torre, Marta; Caballo-González, María; Vico, Eva; Morales-Fernández, Laura; Arriola-Villalobos, Pedro; De las Heras, Beatriz; Benítez-del-Castillo, José M.; Guzmán, Manuel; Millar, Thomas; Herrero-Vanrell, Rocío; Molina-Martínez, Irene T. 2018. "Novel Nano-Liposome Formulation for Dry Eyes with Components Similar to the Preocular Tear Film." Polymers 10, no. 4: 425.
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