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Article

Novel Nano-Liposome Formulation for Dry Eyes with Components Similar to the Preocular Tear Film

1
Pharmaceutical Innovation in Ophthalmology Research Group, UCM 920415, Department of Pharmaceutics and Food Technology, Faculty of Pharmacy, Complutense University of Madrid, Plaza Ramón y Cajal, s/n, 28040 Madrid, Spain
2
Ocular Surface and Inflammation, Ophthalmology Department, San Carlos Clinical Hospital, Calle Profesor Martín Lagos, s/n, 28040 Madrid, Spain
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Sanitary Research Institute of the San Carlos Clinical Hospital (IdISSC) and the Ocular Pathology National Net (OFTARED) of the Institute of Health Carlos III, Calle Profesor Martín Lagos, s/n, 28040 Madrid, Spain
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Department of Pharmacology, Faculty of Pharmacy, Complutense University of Madrid, Plaza Ramón y Cajal, s/n, 28040 Madrid, Spain
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Department of Biomedical Sciences, Faculty of Pharmacy, University of Alcalá, Ctra Madrid-Barcelona, Km 33.6, 28801 Alcalá de Henares, Madrid, Spain
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School of Science and Health, Western Sydney University, Rydalmere Sydney, New South Wales 2116, Australia
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Instituto Universitario de Farmacia Industrial (IUFI), Faculty of Pharmacy, Complutense University, 28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
Polymers 2018, 10(4), 425; https://doi.org/10.3390/polym10040425
Received: 14 March 2018 / Revised: 4 April 2018 / Accepted: 6 April 2018 / Published: 11 April 2018
(This article belongs to the Special Issue Selected Papers from "ECIS 2017")
Dry eye is commonly treated with artificial tears; however, developing artificial tears similar to natural tears is difficult due to the complex nature of tears. We characterized and evaluated a novel artificial tear formulation with components similar to the lipid and aqueous constituents of natural tears. Nano-liposomes, composed in part of phosphatidylcholine, were dispersed in an aqueous solution of bioadhesive sodium hyaluronate. Liposome size, zeta potential, and physicochemical properties of the fresh and stored (4 °C) liposomal formulation were analyzed. In vitro tolerance was tested using human corneal and conjunctival cell lines by exposures of 15 min to 4 h. The tolerance of the liposomal formulation was evaluated in animals (rabbits). The average liposome size was 186.3 ± 7.0 nm, and the zeta potential was negative. The osmolarity of the formulation was 198.6 ± 1.7 mOsm, with a surface tension of 36.5 ± 0.4 mN/m and viscosity of 3.05 ± 0.02 mPa·s. Viability values in the human corneal and conjunctival cell lines were always >80%, even after liposomal formulation storage for 8 weeks. Discomfort and clinical signs after instillation in rabbit eyes were absent. The new formulation, based on phosphatidylcholine-liposomes dispersed in sodium hyaluronate has suitable components and characteristics, including high in vitro cell viability and good in vivo tolerance, to serve as a tear substitute. View Full-Text
Keywords: artificial tears; preocular tear film; tear break-up time; liposomes; mucoadhesive polymers artificial tears; preocular tear film; tear break-up time; liposomes; mucoadhesive polymers
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MDPI and ACS Style

Vicario-de-la-Torre, M.; Caballo-González, M.; Vico, E.; Morales-Fernández, L.; Arriola-Villalobos, P.; De las Heras, B.; Benítez-del-Castillo, J.M.; Guzmán, M.; Millar, T.; Herrero-Vanrell, R.; Molina-Martínez, I.T. Novel Nano-Liposome Formulation for Dry Eyes with Components Similar to the Preocular Tear Film. Polymers 2018, 10, 425. https://doi.org/10.3390/polym10040425

AMA Style

Vicario-de-la-Torre M, Caballo-González M, Vico E, Morales-Fernández L, Arriola-Villalobos P, De las Heras B, Benítez-del-Castillo JM, Guzmán M, Millar T, Herrero-Vanrell R, Molina-Martínez IT. Novel Nano-Liposome Formulation for Dry Eyes with Components Similar to the Preocular Tear Film. Polymers. 2018; 10(4):425. https://doi.org/10.3390/polym10040425

Chicago/Turabian Style

Vicario-de-la-Torre, Marta, María Caballo-González, Eva Vico, Laura Morales-Fernández, Pedro Arriola-Villalobos, Beatriz De las Heras, José M. Benítez-del-Castillo, Manuel Guzmán, Thomas Millar, Rocío Herrero-Vanrell, and Irene T. Molina-Martínez 2018. "Novel Nano-Liposome Formulation for Dry Eyes with Components Similar to the Preocular Tear Film" Polymers 10, no. 4: 425. https://doi.org/10.3390/polym10040425

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