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Polymers 2018, 10(4), 425; https://doi.org/10.3390/polym10040425

Novel Nano-Liposome Formulation for Dry Eyes with Components Similar to the Preocular Tear Film

1
Pharmaceutical Innovation in Ophthalmology Research Group, UCM 920415, Department of Pharmaceutics and Food Technology, Faculty of Pharmacy, Complutense University of Madrid, Plaza Ramón y Cajal, s/n, 28040 Madrid, Spain
2
Ocular Surface and Inflammation, Ophthalmology Department, San Carlos Clinical Hospital, Calle Profesor Martín Lagos, s/n, 28040 Madrid, Spain
3
Sanitary Research Institute of the San Carlos Clinical Hospital (IdISSC) and the Ocular Pathology National Net (OFTARED) of the Institute of Health Carlos III, Calle Profesor Martín Lagos, s/n, 28040 Madrid, Spain
4
Department of Pharmacology, Faculty of Pharmacy, Complutense University of Madrid, Plaza Ramón y Cajal, s/n, 28040 Madrid, Spain
5
Department of Biomedical Sciences, Faculty of Pharmacy, University of Alcalá, Ctra Madrid-Barcelona, Km 33.6, 28801 Alcalá de Henares, Madrid, Spain
6
School of Science and Health, Western Sydney University, Rydalmere Sydney, New South Wales 2116, Australia
7
Instituto Universitario de Farmacia Industrial (IUFI), Faculty of Pharmacy, Complutense University, 28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
Received: 14 March 2018 / Revised: 4 April 2018 / Accepted: 6 April 2018 / Published: 11 April 2018
(This article belongs to the Special Issue Selected Papers from "ECIS 2017")
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Abstract

Dry eye is commonly treated with artificial tears; however, developing artificial tears similar to natural tears is difficult due to the complex nature of tears. We characterized and evaluated a novel artificial tear formulation with components similar to the lipid and aqueous constituents of natural tears. Nano-liposomes, composed in part of phosphatidylcholine, were dispersed in an aqueous solution of bioadhesive sodium hyaluronate. Liposome size, zeta potential, and physicochemical properties of the fresh and stored (4 °C) liposomal formulation were analyzed. In vitro tolerance was tested using human corneal and conjunctival cell lines by exposures of 15 min to 4 h. The tolerance of the liposomal formulation was evaluated in animals (rabbits). The average liposome size was 186.3 ± 7.0 nm, and the zeta potential was negative. The osmolarity of the formulation was 198.6 ± 1.7 mOsm, with a surface tension of 36.5 ± 0.4 mN/m and viscosity of 3.05 ± 0.02 mPa·s. Viability values in the human corneal and conjunctival cell lines were always >80%, even after liposomal formulation storage for 8 weeks. Discomfort and clinical signs after instillation in rabbit eyes were absent. The new formulation, based on phosphatidylcholine-liposomes dispersed in sodium hyaluronate has suitable components and characteristics, including high in vitro cell viability and good in vivo tolerance, to serve as a tear substitute. View Full-Text
Keywords: artificial tears; preocular tear film; tear break-up time; liposomes; mucoadhesive polymers artificial tears; preocular tear film; tear break-up time; liposomes; mucoadhesive polymers
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Vicario-de-la-Torre, M.; Caballo-González, M.; Vico, E.; Morales-Fernández, L.; Arriola-Villalobos, P.; De las Heras, B.; Benítez-del-Castillo, J.M.; Guzmán, M.; Millar, T.; Herrero-Vanrell, R.; Molina-Martínez, I.T. Novel Nano-Liposome Formulation for Dry Eyes with Components Similar to the Preocular Tear Film. Polymers 2018, 10, 425.

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