Anionic Polymer Brushes for Biomimetic Calcium Phosphate Mineralization—A Surface with Application Potential in Biomaterials
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation
2.2. Sample Nomenclature
2.3. Precursors—Prec1, Prec2, Prec3
2.4. ATRP Initiators—Ini1, Ini2, Ini3
2.5. Brushes—Brush1, Brush2, Brush3
2.6. Mineralization—Min1, Min2, Min3
2.7. Atomic Force Microscopy (AFM)
2.8. Contact Angle Measurements
2.9. Scanning Electron Microscopy (SEM)
2.10. Infrared Reflection Absorption Spectroscopy (IRRAS)
2.11. X-Ray Photoelectron Spectroscopy (XPS)
2.12. Cell Culture Experiments
2.13. IRRAS of Brush1, Brush2, Brush3, Min1, Min2, Min3
3. Results
3.1. Polymer Brushes
3.2. Mineralization of Polymer Brushes
3.3. Cell Compatibility of Non-Mineralized and Mineralized Polymer Brushes
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Identifier | Prec1 | Ini1 | Brush1 | Prec2 | Ini2 | Brush2 | Prec3 | Ini3 | Brush3 |
---|---|---|---|---|---|---|---|---|---|
Contact angle (°) | 20 ± 10 | 75 ± 5 | 7 ± 5 | 20 ± 10 | 7 5± 5 | 7 ± 5 | 15 ± 5 | 50 ± 5 | 7 ± 5 |
Thickness by AFM (nm) | 78 ± 6 | 92 ± 10 | 30–300 1 |
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Mai, T.; Wolski, K.; Puciul-Malinowska, A.; Kopyshev, A.; Gräf, R.; Bruns, M.; Zapotoczny, S.; Taubert, A. Anionic Polymer Brushes for Biomimetic Calcium Phosphate Mineralization—A Surface with Application Potential in Biomaterials. Polymers 2018, 10, 1165. https://doi.org/10.3390/polym10101165
Mai T, Wolski K, Puciul-Malinowska A, Kopyshev A, Gräf R, Bruns M, Zapotoczny S, Taubert A. Anionic Polymer Brushes for Biomimetic Calcium Phosphate Mineralization—A Surface with Application Potential in Biomaterials. Polymers. 2018; 10(10):1165. https://doi.org/10.3390/polym10101165
Chicago/Turabian StyleMai, Tobias, Karol Wolski, Agnieszka Puciul-Malinowska, Alexey Kopyshev, Ralph Gräf, Michael Bruns, Szczepan Zapotoczny, and Andreas Taubert. 2018. "Anionic Polymer Brushes for Biomimetic Calcium Phosphate Mineralization—A Surface with Application Potential in Biomaterials" Polymers 10, no. 10: 1165. https://doi.org/10.3390/polym10101165