Self-Assembled Nanostructures of Red Fluorescent Amphiphilic Block Copolymers as Both Imaging Probes and Drug Carriers
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Characterization
2.2. Synthesis of Red Fluorescent Homopolymers: Polycaprolactone (R-PCL) and Polyedecalactone (R-PDL)
2.3. Synthesis of Red-Fluorescent Amphiphilic Block Copolymers (RPO-1–3)
2.4. Preparation of RPO Micellar Structures
2.5. Encapsulation of Doxorubicin (DOX) into RPO-3 Nanoparticles (NPs)
2.6. In Vitro DOX Release with Near-Infrared (NIR) Laser Irradiation
2.7. In Vitro Cellular Uptake of RPO-3 Micelles and DOX@RPO-3 Composite Micelles
2.8. Cytotoxicity of RPO-3 and DOX@RPO-3 NPs
3. Results and Discussion
3.1. Polymer Synthesis and Structural Characterization
3.2. Self-Assembly of RPO-(1–3) in Aqueous Solutions
3.3. Optical Properties of RPO-(1–3) and Their Self-Assembled Nanostructures in Water
3.4. Encapsulation of Anticancer Drugs into RPO Micellar Structures and Controlled Release
3.5. Cellular Internalization of DOX@RPO-3 Nanoparticles and Cytotoxicity
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Entry No. | Polymer Structure | Yield | Mn1 | Mw/Mn |
---|---|---|---|---|
R-PCL-1 | PCL54-dye-PCL54 | 70% | 13,000 | 1.5 |
R-PCL-2 | PCL41-dye-PCL41 | 68% | 10,000 | 1.7 |
R-PDL | PDL22-dye-PDL22 | 77% | 8,000 | 1.7 |
RPO-1 | POEGMA62-b-PCL54-dye-PCL54-b-POEGMA62 | 71% | 50,000 | 1.9 |
RPO-2 | POEGMA17-b-PCL41-dye-PCL41-b-POEGMA17 | 67% | 20,000 | 2.1 |
RPO-3 | POEGMA25-b-PDL22-dye-PDL22-b-POEGMA25 | 40% | 23,000 | 3.3 |
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Huang, S.; Wei, X.; Wang, M. Self-Assembled Nanostructures of Red Fluorescent Amphiphilic Block Copolymers as Both Imaging Probes and Drug Carriers. Polymers 2018, 10, 1120. https://doi.org/10.3390/polym10101120
Huang S, Wei X, Wang M. Self-Assembled Nanostructures of Red Fluorescent Amphiphilic Block Copolymers as Both Imaging Probes and Drug Carriers. Polymers. 2018; 10(10):1120. https://doi.org/10.3390/polym10101120
Chicago/Turabian StyleHuang, Shuo, Xin Wei, and Mingfeng Wang. 2018. "Self-Assembled Nanostructures of Red Fluorescent Amphiphilic Block Copolymers as Both Imaging Probes and Drug Carriers" Polymers 10, no. 10: 1120. https://doi.org/10.3390/polym10101120