Design and Biophysical Characterization of Poly (l-Lactic) Acid Microcarriers with and without Modification of Chitosan and Nanohydroxyapatite
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation and Characterization of PLLA, PLLA/nHA and PLLA/nHA/Ch Microcarriers
2.3. Analysis of Particle Size Distribution
2.4. Morphology Observation and Component Analysis of Microcarriers
2.5. Protein Adsorption Determination
2.6. MC3T3-E1 Cell Culture and Osteogenic Differentiation
2.7. Inoculation and Culture of MC3T3-E1 Cells on Microcarriers
2.8. Cell Adhesion, Proliferation, and Differentiation on Microcarriers
2.9. Statistical Analysis
3. Results and Discussion
3.1. The Particle Size Distribution of Microcarriers
3.2. Morphology, Component Analysis, Protein Adsorption, and Degradation of Microcarriers
3.3. The Morphology and Osteogenic Differentiation of MC3T3-E1 Cells
3.4. Live/Dead Staining of Cells on Microcarriers
3.5. Cell Adhesion, Detachment, and Differentiation on Microcarriers
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Microcarriers | PLLA | PLLA/nHA | PLLA/nHA/Ch |
---|---|---|---|
Mean diameter/μm | 291.9 ± 30.7 | 275.7 ± 30.6 | 269.4 ± 26.3 |
Sphericity | ++ | +++ | + |
Microcarriers | Elemental Fraction (wt %) | ||
---|---|---|---|
C | N | H | |
PLLA | 41.93 ± 0.11 | 0 | 4.80 ± 0.03 |
PLLA/nHA/Ch | 46.96 ± 0.13 | 0.03 ± 0.01 | 5.22 ± 0.01 |
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Li, L.; Song, K.; Chen, Y.; Wang, Y.; Shi, F.; Nie, Y.; Liu, T. Design and Biophysical Characterization of Poly (l-Lactic) Acid Microcarriers with and without Modification of Chitosan and Nanohydroxyapatite. Polymers 2018, 10, 1061. https://doi.org/10.3390/polym10101061
Li L, Song K, Chen Y, Wang Y, Shi F, Nie Y, Liu T. Design and Biophysical Characterization of Poly (l-Lactic) Acid Microcarriers with and without Modification of Chitosan and Nanohydroxyapatite. Polymers. 2018; 10(10):1061. https://doi.org/10.3390/polym10101061
Chicago/Turabian StyleLi, Liying, Kedong Song, Yongzhi Chen, Yiwei Wang, Fangxin Shi, Yi Nie, and Tianqing Liu. 2018. "Design and Biophysical Characterization of Poly (l-Lactic) Acid Microcarriers with and without Modification of Chitosan and Nanohydroxyapatite" Polymers 10, no. 10: 1061. https://doi.org/10.3390/polym10101061