Next Article in Journal
Single Crystal Growth of URu2Si2 by the Modified Bridgman Technique
Next Article in Special Issue
Structural Basis of DEAH/RHA Helicase Activity
Previous Article in Journal
Morphological and Structural Control of Organic Monolayer Colloidal Crystal Based on Plasma Etching and Its Application in Fabrication of Ordered Gold Nanostructured Arrays
Previous Article in Special Issue
3D DNA Crystals and Nanotechnology
Article Menu
Issue 10 (October) cover image

Export Article

Open AccessFeature PaperArticle
Crystals 2016, 6(10), 125;

Phosphorus SAD Phasing for Nucleic Acid Structures: Limitations and Potential

Department of Biochemistry, Vanderbilt University, Nashville TN 37240, USA
Authors to whom correspondence should be addressed.
Academic Editor: Jinwei Zhang
Received: 14 August 2016 / Revised: 12 September 2016 / Accepted: 13 September 2016 / Published: 1 October 2016
(This article belongs to the Special Issue Nucleic Acid Crystallography)
Full-Text   |   PDF [3656 KB, uploaded 1 October 2016]   |  


Phasing of nucleic acid crystal diffraction data using the anomalous signal of phosphorus, P-SAD, at Cukα wavelength has been previously demonstrated using Z-DNA. Since the original work on P-SAD with Z-DNA there has been, with a notable exception, a conspicuous absence of applications of the technique to additional nucleic acid crystal structures. We have reproduced the P-SAD phasing of Z-DNA using a rotating-anode source and have attempted to phase a variety of nucleic acid crystals using P-SAD without success. A comparison of P-SAD using Z-DNA and a representative nucleic acid, the Dickerson-Drew dodecamer, is presented along with a S-SAD using only two sulfurs to phase a 2’-thio modified DNA decamer. A theoretical explanation for the limitation of P-SAD applied to nucleic acids is presented to show that the relatively high atomic displacement parameter of phosphorus in the nucleic acid backbone is responsible for the lack of success in applying P-SAD to nucleic acid diffraction data. View Full-Text
Keywords: SAD phasing; nucleic acid; phosphorus SAD phasing; nucleic acid; phosphorus

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Harp, J.M.; Pallan, P.S.; Egli, M. Phosphorus SAD Phasing for Nucleic Acid Structures: Limitations and Potential. Crystals 2016, 6, 125.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Crystals EISSN 2073-4352 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top