Variations in the Structural and Colloidal Stability of Magnetoferritin under the Impact of Technological Process Modulations

Round 1

Reviewer 1 Report

This interesting manuscript focused on the Iron-based materials, especially magnetite nanocrystals, found extensive applications in many fields.

The authors state that the presented results aid to increase effectiveness of various application of magnetoferritin according to specific industrial requirements.

The results and conclusions are not in doubt. However, the structure part does not have an analysis of the results. Without a deep analysis, it is impossible to find the relationship and understand the nature of the discovered phenomena. This is the main shortcoming of the article. Other minor comments are easy to eliminate. In general, the article may arouse the interest of the reader. Thus, my decision is major revision.

1. Why was nonlinear regression chosen?

2. Why was it necessary to carry out such research in paragraph 2.4? What basic information do they carry? This is not used further in the text of the article.

3. The article does not describe why the absorption increases in Fig. 3.

4. The authors point out that thermal regulation (high sensitivity to cooling and heating of a specific medium) could be a suitable way of changing patterns in thin films and images designed under the influence of a magnetic/electric field. The article does not indicate how this could be.

Author Response

Dear Reviewer,

Please see the attachment

Regards,

Author Response File: Author Response.docx

Reviewer 2 Report

The manuscript will be accepted after a careful polishing of the grammar.

The manuscript will be accepted after a careful polishing of the grammar.

Author Response

Dear Reviewer,

Please see the attachment

Regards,

Author Response File: Author Response.docx

Reviewer 3 Report

Strengths

The authors have this work describes the effect the physico-chemical conditions of the aqueous environment and preparation procedures on the size and protein structure of ferritin derivate, magnetoferritin. They demonstrated various post-synthesis adjustments, such as freezedrying, ultrasonic wave or different solvent use. While the technological synthesis process focuses mainly to obtaining specific nanocrystal types by mineralization of iron precursors during formation of magnetoferritin, the solvent type and viscosity are more significantly associated with colloidal stability. Their results indicate that magnetoferritin sample structure, disrupted by extreme synthesis technological conditions use (temperature or pH), may be restored by suitable solvent viscosity adjustments. The most effective materials with specific requirements for applied research can be prepared after applying different physicochemical synthesis conditions and post-synthesis modifications. Besides practical potential use in biomedicine or nanotechnology, as diagnostics standards, bio sensors, and biological display design, magnetoferritin can show potential in environmen talism in the decontamination methods, in toxins analyses assays or nanocatalysis development.

Good well-coordinated scientific international team. Important promising problems are being solved effectiveness of various application of magnetoferritin according to specific industrial requirements. 

Weakness 

1. 60-70 ^oC, not 60-7 0^oC (Line 148); -55 ^oC, not -55^oC (Line158).

2. Table 1 summarizes the mass concentration of the protein, the LF, not Table 1 summarizes the LF, the mass concentration of the protein (Line 215).

Author Response

Dear Reviewer,

Please see the attachment

Regards,

Author Response File: Author Response.docx

Round 2

Reviewer 1 Report

This interesting manuscript focused on the Iron-based materials, especially magnetite nanocrystals, found extensive applications in many fields.

The authors state that the presented results aid to increase effectiveness of various application of magnetoferritin according to specific industrial requirements.

The authors took into account all comments and revised the article. All comments have been answered. I believe that the article can be published.