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Article

Three-Component Covalent Organic Framework Nanosheets for the Detection of MicroRNAs

by
Bin Xie
1,†,
Yilun Yan
2,†,
Jialin Wu
2,
Xinting Cai
2,
Jiayue Zheng
2,
Yixin Lan
2,
Xihao Tang
2,3,*,
Jun Fan
2,3,
Shengrun Zheng
2,3 and
Songliang Cai
2,3,*
1
Zhuhai Rundu Pharmaceutical Co., Ltd., Zhuhai 519040, China
2
GDMPA Key Laboratory for Process Control and Quality Evaluation of Chiral Pharmaceuticals, School of Chemistry, South China Normal University, Guangzhou 510006, China
3
Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, School of Chemistry, South China Normal University, Guangzhou 510006, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Crystals 2022, 12(11), 1628; https://doi.org/10.3390/cryst12111628
Submission received: 21 October 2022 / Revised: 6 November 2022 / Accepted: 9 November 2022 / Published: 13 November 2022
(This article belongs to the Special Issue Covalent Organic Frameworks)
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Abstract

The development of new techniques for the detection of microRNAs (miRNAs) is highly desirable. Herein, a new crystalline three-component covalent organic framework (COF) termed EB-TAPB-TFP COF was synthesized under solvothermal conditions utilizing 1,3,5-triformylphloroglucinol, 1,3,5-tris(4-aminophenyl)benzene and ethidium bromide as monomers. Interestingly, EB-TAPB-TFP COF can be self-exfoliated into two-dimensional nanosheets (NSs) in an aqueous medium. The obtained EB-TAPB-TFP NSs exhibited a remarkable fluorescence intensity enhancement in the presence of a DNA-miRNA heteroduplex when compared to the presence of single-stranded DNA and other phosphate-based small molecules, making it promising in the detection of miRNA without tagging any fluorescent marker. Moreover, the EB-TAPB-TFP NSs can also be used as sensing material for the detection of a DNA-miRNA heteroduplex using the quartz crystal microbalance technique, which is in good agreement with the fluorescence sensing result. The exploration of COF-based sensors in this work demonstrates a new pathway for the selective detection of miRNAs.
Keywords: covalent organic frameworks; fluorescent detection; microRNAs; quartz crystal microbalance covalent organic frameworks; fluorescent detection; microRNAs; quartz crystal microbalance

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MDPI and ACS Style

Xie, B.; Yan, Y.; Wu, J.; Cai, X.; Zheng, J.; Lan, Y.; Tang, X.; Fan, J.; Zheng, S.; Cai, S. Three-Component Covalent Organic Framework Nanosheets for the Detection of MicroRNAs. Crystals 2022, 12, 1628. https://doi.org/10.3390/cryst12111628

AMA Style

Xie B, Yan Y, Wu J, Cai X, Zheng J, Lan Y, Tang X, Fan J, Zheng S, Cai S. Three-Component Covalent Organic Framework Nanosheets for the Detection of MicroRNAs. Crystals. 2022; 12(11):1628. https://doi.org/10.3390/cryst12111628

Chicago/Turabian Style

Xie, Bin, Yilun Yan, Jialin Wu, Xinting Cai, Jiayue Zheng, Yixin Lan, Xihao Tang, Jun Fan, Shengrun Zheng, and Songliang Cai. 2022. "Three-Component Covalent Organic Framework Nanosheets for the Detection of MicroRNAs" Crystals 12, no. 11: 1628. https://doi.org/10.3390/cryst12111628

APA Style

Xie, B., Yan, Y., Wu, J., Cai, X., Zheng, J., Lan, Y., Tang, X., Fan, J., Zheng, S., & Cai, S. (2022). Three-Component Covalent Organic Framework Nanosheets for the Detection of MicroRNAs. Crystals, 12(11), 1628. https://doi.org/10.3390/cryst12111628

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