Raman Scattering: From Structural Biology to Medical Applications

Vlasov, Alexey V.; Maliar, Nina L.; Bazhenov, Sergey V.; Nikelshparg, Evelina I.; Brazhe, Nadezda A.; Vlasova, Anastasiia D.; Osipov, Stepan D.; Sudarev, Vsevolod V.; Ryzhykau, Yury L.; Bogorodskiy, Andrey O.; Zinovev, Egor V.; Rogachev, Andrey V.; Manukhov, Ilya V.; Borshchevskiy, Valentin I.; Kuklin, Alexander I.; Pokorný, Jan; Sosnovtseva, Olga; Maksimov, Georgy V.; Gordeliy, Valentin I.

doi:10.3390/cryst10010038

Open AccessReview

Raman Scattering: From Structural Biology to Medical Applications

by

Alexey V. Vlasov

^1,2

,

Nina L. Maliar

¹

,

Sergey V. Bazhenov

¹

,

Evelina I. Nikelshparg

³,

Nadezda A. Brazhe

³,

Anastasiia D. Vlasova

¹,

Stepan D. Osipov

¹,

Vsevolod V. Sudarev

¹,

Yury L. Ryzhykau

¹

,

Andrey O. Bogorodskiy

¹,

Egor V. Zinovev

^1,2,4,

Andrey V. Rogachev

^1,5,

Ilya V. Manukhov

¹,

Valentin I. Borshchevskiy

^1,4,6,

Alexander I. Kuklin

^1,5,

Jan Pokorný

⁷,

Olga Sosnovtseva

⁸,

Georgy V. Maksimov

³ and

Valentin I. Gordeliy

^1,4,6,9,*

¹

Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Russia

²

Institute of Crystallography, RWTH Aachen University, 52066 Aachen, Germany

³

Biophysics Department, Biological Faculty, Moscow State University, 119234 Moscow, Russia

⁴

Institute of Complex Systems (ICS), ICS-6: Structural Biochemistry, Research Centre Jülich, 52425 Jülich, Germany

⁵

Joint Institute for Nuclear Research, 141980 Dubna, Russia

⁶

JuStruct: Jülich Center for Structural Biology, Forschungszentrum Jülich, 52425 Jülich, Germany

⁷

Institute of Physics of the Czech Academy of Sciences, 18221 Prague, Czech Republic

⁸

Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark

⁹

Institut de Biologie Structurale Jean-Pierre Ebel, Université Grenoble Alpes—Commissariat à l’Energie Atomique et aux Energies Alternatives—CNRS, 38044 Grenoble, France

^*

Author to whom correspondence should be addressed.

Crystals 2020, 10(1), 38; https://doi.org/10.3390/cryst10010038

Submission received: 9 December 2019 / Revised: 9 January 2020 / Accepted: 10 January 2020 / Published: 15 January 2020

(This article belongs to the Special Issue Raman Spectroscopy of Crystals)

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Abstract

This is a review of relevant Raman spectroscopy (RS) techniques and their use in structural biology, biophysics, cells, and tissues imaging towards development of various medical diagnostic tools, drug design, and other medical applications. Classical and contemporary structural studies of different water-soluble and membrane proteins, DNA, RNA, and their interactions and behavior in different systems were analyzed in terms of applicability of RS techniques and their complementarity to other corresponding methods. We show that RS is a powerful method that links the fundamental structural biology and its medical applications in cancer, cardiovascular, neurodegenerative, atherosclerotic, and other diseases. In particular, the key roles of RS in modern technologies of structure-based drug design are the detection and imaging of membrane protein microcrystals with the help of coherent anti-Stokes Raman scattering (CARS), which would help to further the development of protein structural crystallography and would result in a number of novel high-resolution structures of membrane proteins—drug targets; and, structural studies of photoactive membrane proteins (rhodopsins, photoreceptors, etc.) for the development of new optogenetic tools. Physical background and biomedical applications of spontaneous, stimulated, resonant, and surface- and tip-enhanced RS are also discussed. All of these techniques have been extensively developed during recent several decades. A number of interesting applications of CARS, resonant, and surface-enhanced Raman spectroscopy methods are also discussed.

Keywords: Raman scattering; SERS; biophysics; structural biology; DNA; cancer; cell imaging; medical applications; hemoproteins; photoactive proteins

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MDPI and ACS Style

Vlasov, A.V.; Maliar, N.L.; Bazhenov, S.V.; Nikelshparg, E.I.; Brazhe, N.A.; Vlasova, A.D.; Osipov, S.D.; Sudarev, V.V.; Ryzhykau, Y.L.; Bogorodskiy, A.O.; et al. Raman Scattering: From Structural Biology to Medical Applications. Crystals 2020, 10, 38. https://doi.org/10.3390/cryst10010038

AMA Style

Vlasov AV, Maliar NL, Bazhenov SV, Nikelshparg EI, Brazhe NA, Vlasova AD, Osipov SD, Sudarev VV, Ryzhykau YL, Bogorodskiy AO, et al. Raman Scattering: From Structural Biology to Medical Applications. Crystals. 2020; 10(1):38. https://doi.org/10.3390/cryst10010038

Chicago/Turabian Style

Vlasov, Alexey V., Nina L. Maliar, Sergey V. Bazhenov, Evelina I. Nikelshparg, Nadezda A. Brazhe, Anastasiia D. Vlasova, Stepan D. Osipov, Vsevolod V. Sudarev, Yury L. Ryzhykau, Andrey O. Bogorodskiy, and et al. 2020. "Raman Scattering: From Structural Biology to Medical Applications" Crystals 10, no. 1: 38. https://doi.org/10.3390/cryst10010038

APA Style

Vlasov, A. V., Maliar, N. L., Bazhenov, S. V., Nikelshparg, E. I., Brazhe, N. A., Vlasova, A. D., Osipov, S. D., Sudarev, V. V., Ryzhykau, Y. L., Bogorodskiy, A. O., Zinovev, E. V., Rogachev, A. V., Manukhov, I. V., Borshchevskiy, V. I., Kuklin, A. I., Pokorný, J., Sosnovtseva, O., Maksimov, G. V., & Gordeliy, V. I. (2020). Raman Scattering: From Structural Biology to Medical Applications. Crystals, 10(1), 38. https://doi.org/10.3390/cryst10010038

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