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Open AccessArticle

An Improved Method to Encapsulate Laccase from Trametes versicolor with Enhanced Stability and Catalytic Activity

1,†, 1,†, 1,* and 2,*
1
College of Life Science, Jilin Agricultural University, Changchun 130118, China
2
Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, Changchun 130012, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Catalysts 2018, 8(7), 286; https://doi.org/10.3390/catal8070286
Received: 14 June 2018 / Revised: 5 July 2018 / Accepted: 12 July 2018 / Published: 17 July 2018
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PDF [1698 KB, uploaded 17 July 2018]
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Abstract

In this work, laccase from Trametes versicolor pretreated with copper ion solution was entrapped in copper alginate beads. The presence of laccase in copper alginate beads was verified by Fourier transform infrared (FTIR) spectroscopy. The alginate concentration used was optimized based on the specific activity and immobilization yield. After entrapment, laccase presents perfect pH stability and thermal stability with 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) as the substrate. Moreover, laccase in copper alginate beads exhibits good reusability during continuous batch operation for removing 2,4-dichlorophenol. More importantly, owing to the coupled effect of copper ion activation and copper alginate entrapment, the entrapped laccase shows a 3.0-fold and a 2.4-fold increase in specific activity and 2,4-DCP degradation rate compared with that of free laccase, respectively. View Full-Text
Keywords: laccase; copper alginate; entrapment; specific activity; degradation rate laccase; copper alginate; entrapment; specific activity; degradation rate
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Zhang, S.; Wu, Z.; Chen, G.; Wang, Z. An Improved Method to Encapsulate Laccase from Trametes versicolor with Enhanced Stability and Catalytic Activity. Catalysts 2018, 8, 286.

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