Next Article in Journal
Immobilization of Prunus amygdalus Hydroxynitrile Lyase on Celite
Previous Article in Journal
Ag/CeO2 Composites for Catalytic Abatement of CO, Soot and VOCs
Open AccessArticle

An Improved Method to Encapsulate Laccase from Trametes versicolor with Enhanced Stability and Catalytic Activity

by 1,†, 1,†, 1,* and 2,*
1
College of Life Science, Jilin Agricultural University, Changchun 130118, China
2
Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, Changchun 130012, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Catalysts 2018, 8(7), 286; https://doi.org/10.3390/catal8070286
Received: 14 June 2018 / Revised: 5 July 2018 / Accepted: 12 July 2018 / Published: 17 July 2018
In this work, laccase from Trametes versicolor pretreated with copper ion solution was entrapped in copper alginate beads. The presence of laccase in copper alginate beads was verified by Fourier transform infrared (FTIR) spectroscopy. The alginate concentration used was optimized based on the specific activity and immobilization yield. After entrapment, laccase presents perfect pH stability and thermal stability with 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) as the substrate. Moreover, laccase in copper alginate beads exhibits good reusability during continuous batch operation for removing 2,4-dichlorophenol. More importantly, owing to the coupled effect of copper ion activation and copper alginate entrapment, the entrapped laccase shows a 3.0-fold and a 2.4-fold increase in specific activity and 2,4-DCP degradation rate compared with that of free laccase, respectively. View Full-Text
Keywords: laccase; copper alginate; entrapment; specific activity; degradation rate laccase; copper alginate; entrapment; specific activity; degradation rate
Show Figures

Graphical abstract

MDPI and ACS Style

Zhang, S.; Wu, Z.; Chen, G.; Wang, Z. An Improved Method to Encapsulate Laccase from Trametes versicolor with Enhanced Stability and Catalytic Activity. Catalysts 2018, 8, 286.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map

1
Back to TopTop