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Electrochemical Biosensor for the Determination of Amlodipine Besylate Based on Gelatin–Polyaniline Iron Oxide Biocomposite Film

Laboratory of Environmental Engineering, Department of Process Engineering, Faculty of Engineering Sciences, Badji Mokhtar University-Annaba, P.O. Box 12, 23000 Annaba, Algeria
Author to whom correspondence should be addressed.
Catalysts 2018, 8(6), 233;
Received: 19 April 2018 / Revised: 10 May 2018 / Accepted: 18 May 2018 / Published: 4 June 2018
(This article belongs to the Special Issue Biocatalysts: Design and Application)
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In the present study, a new biosensor based on lipase from Candida rugosa (CRL) was developed for amlodipine besylate drug (AMD) with biodegradable material using a mixture of polyaniline iron oxide and gelatin. Polyaniline/Fe2O3 ([email protected]2O3) was prepared by a chemical polymerization method in a medium of ammonium persulfate as an oxidant and characterized by employing Scanning Electron Microscopy (SEM), Fourier Transform Infrared (FTIR), and Ultra-violet (UV) spectroscopy. The purified enzyme was entrapped in the biocomposite matrix film with the aid of a glutaraldehyde cross-linking reagent to establish the immobilization of the lipase. The principle of the biosensor is based on the electrochemical properties of amlodipine besylate (AMD), which were studied for the first time using the cyclic voltammetric method. The cathodic behavior of AMD was measured on the irreversible reduction signal at −0.185 V versus Ag/AgCl at pH 7.4 and 30 °C in a phosphate alkaline buffer. View Full-Text
Keywords: biosensors; lipase; amlodipine; polyaniline; cyclic voltammetry biosensors; lipase; amlodipine; polyaniline; cyclic voltammetry

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Djaalab, E.; Samar, M.E.H.; Zougar, S.; Kherrat, R. Electrochemical Biosensor for the Determination of Amlodipine Besylate Based on Gelatin–Polyaniline Iron Oxide Biocomposite Film. Catalysts 2018, 8, 233.

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