Identification of New Dioxygenases Able to Recognize Polycyclic Aromatic Hydrocarbons with High Aromaticity
Round 1
Reviewer 1 Report
Comments for Authors
- Write keywords in alphabetical order.
- The authors want to put more latest related citations in the introduction part.
- Use EndNote or Mendeley software for references sequences.
- For %, °C, wt % and for Figure, etc., follow the same format throughout the manuscript.
- Check grammatically and spelling throughout the manuscript. There are so many mistakes.
- Figure 7 does not give much more information regarding aromatic rings. Revise it with more explanations.
- Results and Discussion; The same study was carried out with another biphenyl dioxygenase from Para-116 burkholderia xenovorans (PDB ID 2xsh) which has similar molecular surface area. [Give the specific reference and more explanations]
Cite the following references;
- doi:10.1007/s10924-021-02142-1
- doi: 10.3390/polym13020268.
- https://doi.org/10.1007/s42235-021-0046-7
Author Response
Review 1
- 1) Write keywords in alphabetical order.
answer: Keywords are written in alphabetical order.
2) The authors want to put more latest related citations in the introduction part
answer: The bibliography has been updated with more recent references.
3) Use EndNote or Mendeley software for references sequences
answer: We have used Mendeley software for references sequences.
- 4) For %, °C, wt % and for Figure, etc., follow the same format throughout the manuscript.
answer: We have followed the same format throughout the manuscript for symbols and Figures.
5) Check grammatically and spelling throughout the manuscript. There are so many mistakes
answer: We have submit the revised version of the manuscript to the English Editing Services
6) Review: Figure 7 does not give much more information regarding aromatic rings.
Answer: Figure 6 present binding mode of all PAHs used in this paper (the structural conformation of the compounds is not very well appreciated), while Figure 7 shows the binding mode of the two PAHs with greater aromaticity (benzo[a]pyrene and indeno(1,2,3-cd)pyrene, compounds hardly recognized by other dioxygenases) as well as their interactions with the key residues of the catalytic center. More information regarding this figure has been written in the communication.
- Results and Discussion; The same study was carried out with another biphenyl dioxygenase from Para-116 burkholderia xenovorans (PDB ID 2xsh) which has similar molecular surface area. [Give the specific reference and more explanations]
Answer: The same study was carried out with another biphenyl dioxygenase from Paraburkholderia xenovorans (as well known as Burholderia xenovorans PDB ID 2xsh) which has similar molecular surface area into binding pocket to the biphenyl dioxygenase from Sphingobium yanoikuyae (calculated by Maestro, 421.550 Å2). In addition, the amino acid that form the catalytic pocket are very similar in both enzymes (phenylalanines and histidines). It enzyme cometabolizes many polychlorinated biphenyl compounds and have become a model system for the bacterial breakdown of these very persistent environmental contaminants [26].
8) Cite the following references;
- doi:10.1007/s10924-021-02142-1
- doi: 10.3390/polym13020268.
- https://doi.org/10.1007/s42235-021-0046-7
I have not added the following references proposed by the reviewer as they are not closely related to the subject.
- doi:10.1007/s10924-021-02142-1-
Title: Recent Progress in Silane Coupling Agent with Its Emerging Applications
- doi: 10.3390/polym13020268.
Title: Kinetic and Thermal Study of Ethylene and Propylene Homo Polymerization Catalyzed by ansa-Zirconocene Activated with Alkylaluminum/Borate: Effects of Alkylaluminum on Polymerization Kinetics and Polymer Structure
- https://doi.org/10.1007/s42235-021-0046-7
Title: Triple-Scale Superhydrophobic Surface with Excellent Anti-Icing and Icephobic Performance via Ultrafast Laser Hybrid Fabrication
Author Response File: 
Author Response.docx
Reviewer 2 Report
This communication presents promising results about HAP bioremediation even if these researches are not totally new.
This paper could be improved by adding some information concerning the selection of aromatic compounds and why biphenyl was not included in the study while it is the natural substrat of these enzymes.
The Figures S1 is not useful as the same content is already given in the text.
Author Response
Review 2
Comments and Suggestions for Authors
This communication presents promising results about HAP bioremediation even if these researches are not totally new.
- This paper could be improved by adding some information concerning the selection of aromatic compounds and why biphenyl was not included in the study while it is the natural substrate of these enzymes.
Answer: We have chosen these PAHs because they are included in the list of United States Environmental protection Agency (EPA) as Priority Study Contaminants. Biphenyl is not included in that list because its chemical-physical properties (solubility and recalcitrance) are not as bad as those of the others.
.
- The Figures S1 is not useful as the same content is already given in the text.
Answer: Figure S1 has been removed from support info.
Thanks to the reviewer for their work and comments to improve the communication.
Best regards,
Agatha Bastida (24-02-2022)
Author Response File: Author Response.docx
