Co-Enzymes with Dissimilar Stabilities: A Discussion of the Likely Biocatalyst Performance Problems and Some Potential Solutions
Abstract
:1. Introduction
2. Long-Term Performance of a Combi-Biocatalyst using Enzymes with Dissimilar Stabilities
2.1. Enzyme Deactivation and Kinetics
2.2. Description of the Case Study
3. Effect of Enzyme Inactivation when the Combined Use of Enzymes Only Increases the Volumetric Activity of the Combi-Biocatalysts
- (1)
- Both enzymes deactivate at a similar rate.
- (2)
- The first enzyme in the cascade deactivates, while the second fully retains its activity.
- (3)
- The second enzyme in the cascade deactivates, while the first fully retains its activity.
3.1. Effect of Enzyme Deactivation on Biocatalyst Performance
3.1.1. Both Enzymes Deactivate at a Similar Rate
3.1.2. Enzyme 1 Deactivates while Enzyme 2 Remains Fully Active
3.1.3. Enzyme 2 Deactivates while Enzyme 1 Remains Fully Active
3.2. Strategies to Maintain a Similar Reaction Course during the 50 Cycles
3.3. Use of Co-Immobilized Enzymes
4. Design of Combi-Biocatalysts: Strategies to Improve Biocatalyst Performance and Reuse the More Stable Enzyme
5. Concluding Remarks
Author Contributions
Funding
Conflicts of Interest
References
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Parameter | Enzyme 1 | Enzyme 2 |
---|---|---|
4 µM/s | 6 µM/s | |
2 mM | 4 mM | |
1·10−3 s−1 | 2·10−3 s−1 | |
4 mM | 6 mM |
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Høst, A.V.; Morellon-Sterling, R.; Carballares, D.; Woodley, J.M.; Fernandez-Lafuente, R. Co-Enzymes with Dissimilar Stabilities: A Discussion of the Likely Biocatalyst Performance Problems and Some Potential Solutions. Catalysts 2022, 12, 1570. https://doi.org/10.3390/catal12121570
Høst AV, Morellon-Sterling R, Carballares D, Woodley JM, Fernandez-Lafuente R. Co-Enzymes with Dissimilar Stabilities: A Discussion of the Likely Biocatalyst Performance Problems and Some Potential Solutions. Catalysts. 2022; 12(12):1570. https://doi.org/10.3390/catal12121570
Chicago/Turabian StyleHøst, Amalie Vang, Roberto Morellon-Sterling, Diego Carballares, John M. Woodley, and Roberto Fernandez-Lafuente. 2022. "Co-Enzymes with Dissimilar Stabilities: A Discussion of the Likely Biocatalyst Performance Problems and Some Potential Solutions" Catalysts 12, no. 12: 1570. https://doi.org/10.3390/catal12121570