Recombinant Production of Arginyl Dipeptides by l-Amino Acid Ligase RizA Coupled with ATP Regeneration
Abstract
:1. Introduction
2. Results and Discussion
2.1. Influence of Substrate Concentrations
2.2. Influence of Cofactor Concentrations
2.3. Influence of Enzyme Concentrations
2.4. Influence of Temperature and pH
2.5. Time Course of Formation of Arg-Ser, Arg-Ala, Arg-Gly, and Arg-Arg
3. Materials and Methods
3.1. Chemicals, Reagents and Strains
3.2. Construction of pET28a_his6-rizA and pET28a_his6-ackA Constructs
3.3. Cultivation and Expression
3.4. Purification
3.5. Biocatalysis
3.6. Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Bordewick, S.; Mast, T.A.; Berger, R.G.; Ersoy, F. Recombinant Production of Arginyl Dipeptides by l-Amino Acid Ligase RizA Coupled with ATP Regeneration. Catalysts 2021, 11, 1290. https://doi.org/10.3390/catal11111290
Bordewick S, Mast TA, Berger RG, Ersoy F. Recombinant Production of Arginyl Dipeptides by l-Amino Acid Ligase RizA Coupled with ATP Regeneration. Catalysts. 2021; 11(11):1290. https://doi.org/10.3390/catal11111290
Chicago/Turabian StyleBordewick, Sven, Tim A. Mast, Ralf G. Berger, and Franziska Ersoy. 2021. "Recombinant Production of Arginyl Dipeptides by l-Amino Acid Ligase RizA Coupled with ATP Regeneration" Catalysts 11, no. 11: 1290. https://doi.org/10.3390/catal11111290