Recombinant Production of Arginyl Dipeptides by l-Amino Acid Ligase RizA Coupled with ATP Regeneration
Institut für Lebensmittelchemie, Gottfried Wilhelm Leibniz Universität Hannover, Callinstraße 5, 30167 Hannover, Germany
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Academic Editors: Edinson Yara-Varón and Ramon Canela-Garayoa
Catalysts 2021, 11(11), 1290; https://doi.org/10.3390/catal11111290
Received: 30 September 2021
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Revised: 22 October 2021
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Accepted: 25 October 2021
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Published: 27 October 2021
(This article belongs to the Special Issue Enzyme Catalysis, Biotransformation and Bioeconomy)
Arginyl dipeptides like Arg-Ser, Arg-Ala, and Arg-Gly are salt-taste enhancers and can potentially be used to reduce the salt content of food. The l-amino acid ligase RizA from B. subtilis selectively synthesizes arginyl dipeptides. However, industrial application is prevented by the high cost of the cofactor adenosine triphosphate (ATP). Thus, a coupled reaction system was created consisting of RizA and acetate kinase (AckA) from E. coli providing ATP regeneration from acetyl phosphate. Both enzymes were recombinantly produced in E. coli and purified by affinity chromatography. Biocatalytic reactions were varied and analyzed by RP-HPLC with fluorescence detection. Under optimal conditions the system produced up to 5.9 g/L Arg-Ser corresponding to an ATP efficiency of 23 g Arg-Ser per gram ATP. Using similar conditions with alanine or glycine as second amino acid, 2.6 g/L Arg-Ala or 2.4 g/L Arg Gly were produced. The RizA/AckA system selectively produced substantial amounts of arginyl dipeptides while minimizing the usage of the expensive ATP.
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Keywords:
l-amino acid ligase; acetate kinase; coupled catalysis; arginyl dipeptides; salt taste; ATP regeneration
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MDPI and ACS Style
Bordewick, S.; Mast, T.A.; Berger, R.G.; Ersoy, F. Recombinant Production of Arginyl Dipeptides by l-Amino Acid Ligase RizA Coupled with ATP Regeneration. Catalysts 2021, 11, 1290. https://doi.org/10.3390/catal11111290
AMA Style
Bordewick S, Mast TA, Berger RG, Ersoy F. Recombinant Production of Arginyl Dipeptides by l-Amino Acid Ligase RizA Coupled with ATP Regeneration. Catalysts. 2021; 11(11):1290. https://doi.org/10.3390/catal11111290
Chicago/Turabian StyleBordewick, Sven, Tim A. Mast, Ralf G. Berger, and Franziska Ersoy. 2021. "Recombinant Production of Arginyl Dipeptides by l-Amino Acid Ligase RizA Coupled with ATP Regeneration" Catalysts 11, no. 11: 1290. https://doi.org/10.3390/catal11111290
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