Synthesis and Characterization of Bio-Active GFP-P4VP Core–Shell Nanoparticles
Department of Chemistry and Biochemistry, Northern Illinois University, Dekalb, IL 60115, USA
Center for Nanoscale Materials, Argonne National Laboratory, Lemont, IL 60439, USA
Neuqua Valley High School, Naperville, IL 60564, USA
X-ray Science Division, Argonne National Laboratory, Lemont, IL 60439, USA
Author to whom correspondence should be addressed.
Catalysts 2020, 10(6), 627; https://doi.org/10.3390/catal10060627
Received: 4 May 2020 / Revised: 27 May 2020 / Accepted: 3 June 2020 / Published: 5 June 2020
(This article belongs to the Special Issue Bio-Inspired Materials: Learning from the Nature to Improve the Catalyst)
Bioactive core–shell nanoparticles (CSNPs) offer the unique ability for protein/enzyme functionality in non-native environments. For many decades, researchers have sought to develop synthetic materials which mimic the efficiency and catalytic power of bioactive macromolecules such as enzymes and proteins. This research studies a self-assembly method in which functionalized, polymer-core/protein-shell nanoparticles are prepared in mild conditions. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) techniques were utilized to analyze the size and distribution of the CSNPs. The methods outlined in this research demonstrate a mild, green chemistry synthesis route for CSNPs which are highly tunable and allow for enzyme/protein functionality in non-native conditions.