Rapamycin-Induced Feedback Activation of eIF4E-EIF4A Dependent mRNA Translation in Pancreatic Cancer
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. Ribosome Footprinting Identifies Translational Targets of mTOR and RPS6 (S6)
2.2. Rapamycin Inhibits the Translation of Genes Involved in the Cell Cycle and Cancer Cell Growth
2.3. Rapamycin Induces Translation of a Subset of mRNAs
2.4. Rapamycin Activates Translation through Feedback Activation of AKT1 and eIF4E
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Treatments
4.2. Ribosome Footprinting
4.3. Sequence Alignment
4.4. Footprint Profile Analysis
4.5. Metagene Analysis
4.6. Global mRNA Translation
4.7. Cell Viability Assay
4.8. Clonogenic Survival Assay
4.9. Immunoblotting
4.10. Luciferase Reporter Assay
4.11. Statistical Analysis
4.12. Online Content
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Nguyen, T.U.; Hector, H.; Pederson, E.N.; Lin, J.; Ouyang, Z.; Wendel, H.-G.; Singh, K. Rapamycin-Induced Feedback Activation of eIF4E-EIF4A Dependent mRNA Translation in Pancreatic Cancer. Cancers 2023, 15, 1444. https://doi.org/10.3390/cancers15051444
Nguyen TU, Hector H, Pederson EN, Lin J, Ouyang Z, Wendel H-G, Singh K. Rapamycin-Induced Feedback Activation of eIF4E-EIF4A Dependent mRNA Translation in Pancreatic Cancer. Cancers. 2023; 15(5):1444. https://doi.org/10.3390/cancers15051444
Chicago/Turabian StyleNguyen, Trang Uyen, Harrison Hector, Eric Nels Pederson, Jianan Lin, Zhengqing Ouyang, Hans-Guido Wendel, and Kamini Singh. 2023. "Rapamycin-Induced Feedback Activation of eIF4E-EIF4A Dependent mRNA Translation in Pancreatic Cancer" Cancers 15, no. 5: 1444. https://doi.org/10.3390/cancers15051444