In Situ Imaging of O-Linked β-N-Acetylglucosamine Using On-Tissue Hydrolysis and MALDI Mass Spectrometry
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
3. Materials & Methods
3.1. Chemicals
3.2. Sample Preparation & Analysis
3.3. LC-MS/MS Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Escobar, E.E.; Seeley, E.H.; Serrano-Negrón, J.E.; Vocadlo, D.J.; Brodbelt, J.S. In Situ Imaging of O-Linked β-N-Acetylglucosamine Using On-Tissue Hydrolysis and MALDI Mass Spectrometry. Cancers 2023, 15, 1224. https://doi.org/10.3390/cancers15041224
Escobar EE, Seeley EH, Serrano-Negrón JE, Vocadlo DJ, Brodbelt JS. In Situ Imaging of O-Linked β-N-Acetylglucosamine Using On-Tissue Hydrolysis and MALDI Mass Spectrometry. Cancers. 2023; 15(4):1224. https://doi.org/10.3390/cancers15041224
Chicago/Turabian StyleEscobar, Edwin E., Erin H. Seeley, Jesús E. Serrano-Negrón, David J. Vocadlo, and Jennifer S. Brodbelt. 2023. "In Situ Imaging of O-Linked β-N-Acetylglucosamine Using On-Tissue Hydrolysis and MALDI Mass Spectrometry" Cancers 15, no. 4: 1224. https://doi.org/10.3390/cancers15041224