SPRY4 as a Potential Mediator of the Anti-Tumoral Role of Macrophages in Anaplastic Thyroid Cancer Cells
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Lines
2.2. Macrophages
2.3. Macrophage–ATC Co-Cultures
2.4. Transient Inhibition of SPRY4 Using siRNA-Mediated Silencing
2.5. Metabolic Cell Viability and Cytotoxic Drug Assay
2.6. Migration and Invasion Assays
2.7. Actin Filament Staining
2.8. Flow Cytometry
2.9. Conditioned Medium Analysis
2.10. MS-Based Proteomics
2.11. Western Blot Validation
2.12. Statistical Analysis
3. Results
3.1. Macrophages Decrease the Viability of T238 and C3948 ATC Cell Lines, While Maintaining the Intrinsic Chemoresistance Profile of Cancer Cells
3.2. Macrophages Increase the Migration Ability of T235 ATC Cell Lines, While Decreasing That of T238 and C3948
3.3. Macrophages Increase the Invasion Ability of T235 and C643 ATC Cell Lines, While Decreasing That of C3948
3.4. Co-Culture with Macrophages Affects Differently ATC Cell Cytoskeleton
3.5. Co-Culture of Macrophages with C3948 or T235 Exhibit a Different Cytokine Profile
3.6. The Inflammatory Profile of Macrophages Is Different in Co-Culture with C3948 or T235 Cells
3.7. Co-Culture with Macrophages Differentially Deregulates the Proteome Profile of C3948 and T235 Cells Unveiling SPRY4 as a Differentially Expressed Protein
3.8. SPRY4 Silencing Reverts the Macrophage-Induced Reduction of C3948 Cell’s Invasion and Promotes Cytoskeleton Alterations in ATC Cells
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Pinto, A.T.; Pojo, M.; Rodrigues, R.; Sousa, D.P.; Matthiesen, R.; Carvalho, A.S.; Beck, H.C.; Pires, C.; Eduardo, R.; Pereira, J.S.; et al. SPRY4 as a Potential Mediator of the Anti-Tumoral Role of Macrophages in Anaplastic Thyroid Cancer Cells. Cancers 2023, 15, 4387. https://doi.org/10.3390/cancers15174387
Pinto AT, Pojo M, Rodrigues R, Sousa DP, Matthiesen R, Carvalho AS, Beck HC, Pires C, Eduardo R, Pereira JS, et al. SPRY4 as a Potential Mediator of the Anti-Tumoral Role of Macrophages in Anaplastic Thyroid Cancer Cells. Cancers. 2023; 15(17):4387. https://doi.org/10.3390/cancers15174387
Chicago/Turabian StylePinto, Ana Teresa, Marta Pojo, Ricardo Rodrigues, Diana Pacheco Sousa, Rune Matthiesen, Ana Sofia Carvalho, Hans C. Beck, Carolina Pires, Rodrigo Eduardo, Joana Simões Pereira, and et al. 2023. "SPRY4 as a Potential Mediator of the Anti-Tumoral Role of Macrophages in Anaplastic Thyroid Cancer Cells" Cancers 15, no. 17: 4387. https://doi.org/10.3390/cancers15174387