Functional In Vivo Screening Identifies microRNAs Regulating Metastatic Dissemination of Prostate Cancer Cells to Bone Marrow
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Patient Samples
2.2. Cell Lines
2.3. RNA Extraction, Reverse Transcription and qRT-PCR
2.4. Transduction of Cells with miRZiP Library
2.5. In Vivo PCa Model of Metastatic Migration
2.6. DNA Extraction and PCR
2.7. DNA Sequencing
2.8. Immunohistochemical Analysis
2.9. RNA Immunoprecipitation Sequencing (RIP-Seq)
2.10. RNA Sequencing
2.11. In Vitro Functional Analyses
2.12. Data Processing and Statistical Analysis
3. Results
3.1. Transduction of PCa Cells with Anti-miR Library
3.2. In Vivo Functional Library Screening
3.3. Metastatic Dissemination
3.4. In Vitro Functional Analyses
3.5. Metastatic Dissemination to Bone
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
miRNA | microRNA |
PCa | Prostate cancer |
OB-CM | Osteoblast-conditioned medium |
GFP | green fluorescent protein |
IP | immunoprecipitation |
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Ivkovic, T.C.; Cornella, H.; Voss, G.; Ku, A.; Persson, M.; Rigo, R.; Gruvberger-Saal, S.K.; Saal, L.H.; Ceder, Y. Functional In Vivo Screening Identifies microRNAs Regulating Metastatic Dissemination of Prostate Cancer Cells to Bone Marrow. Cancers 2023, 15, 3892. https://doi.org/10.3390/cancers15153892
Ivkovic TC, Cornella H, Voss G, Ku A, Persson M, Rigo R, Gruvberger-Saal SK, Saal LH, Ceder Y. Functional In Vivo Screening Identifies microRNAs Regulating Metastatic Dissemination of Prostate Cancer Cells to Bone Marrow. Cancers. 2023; 15(15):3892. https://doi.org/10.3390/cancers15153892
Chicago/Turabian StyleIvkovic, Tina Catela, Helena Cornella, Gjendine Voss, Anson Ku, Margareta Persson, Robert Rigo, Sofia K. Gruvberger-Saal, Lao H. Saal, and Yvonne Ceder. 2023. "Functional In Vivo Screening Identifies microRNAs Regulating Metastatic Dissemination of Prostate Cancer Cells to Bone Marrow" Cancers 15, no. 15: 3892. https://doi.org/10.3390/cancers15153892