Identification, and Experimental and Bioinformatics Validation of an Immune-Related Prognosis Gene Signature for Low-Grade Glioma Based on mRNAsi
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. LGG and Perineural Tissue Acquisition
2.2. Collecting Immune-Related Genes and Datasets
2.3. Calculation of the mRNA Stemness Index
2.4. WGCNA to Filter Key Module
2.5. Determination of Immune-Related Genes with Differential Expression
2.6. Hub Gene Identification
2.7. Identification of Hub Genes
2.8. Chemotherapy Sensitivity Response Predictions
2.9. Translation Level of Hub Gene Expression Identification
2.10. Prognostic Risk System Establishment
2.11. Multivariate Cox Regression Analysis
2.12. Nomogram Construction and Verification
2.13. Prognostic Risk Signature Functional Exploration
2.14. Relevance between Hub Genes’ Expression and Immune Cells
2.15. qPCR
2.16. Statistical Analysis
3. Results
3.1. Key Module Identification
3.2. Hub Gene Screening
3.3. Screening for Potential Prognostic Genes
3.4. Potential Function of DEGs
3.5. Verification of Hub Gene Expression in Normal Tissues and LGG Tissues
3.6. IPS Building
3.7. Clinical Nomogram Based on Created Immune-Related Prognostic Signature
3.8. GSEA Analysis
3.9. Correlation between Immune Infiltration and IPS in LGG
3.10. Predicting Immunotherapy Response
3.11. Validation of Hub Genes’ Expression
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wang, Y.; Ye, S.; Wu, D.; Xu, Z.; Wei, W.; Duan, F.; Luo, M. Identification, and Experimental and Bioinformatics Validation of an Immune-Related Prognosis Gene Signature for Low-Grade Glioma Based on mRNAsi. Cancers 2023, 15, 3238. https://doi.org/10.3390/cancers15123238
Wang Y, Ye S, Wu D, Xu Z, Wei W, Duan F, Luo M. Identification, and Experimental and Bioinformatics Validation of an Immune-Related Prognosis Gene Signature for Low-Grade Glioma Based on mRNAsi. Cancers. 2023; 15(12):3238. https://doi.org/10.3390/cancers15123238
Chicago/Turabian StyleWang, Yuan, Shengda Ye, Du Wu, Ziyue Xu, Wei Wei, Faliang Duan, and Ming Luo. 2023. "Identification, and Experimental and Bioinformatics Validation of an Immune-Related Prognosis Gene Signature for Low-Grade Glioma Based on mRNAsi" Cancers 15, no. 12: 3238. https://doi.org/10.3390/cancers15123238