CENPE Inhibition Leads to Mitotic Catastrophe and DNA Damage in Medulloblastoma Cells
Neuroscience Institute Cavalieri Ottolenghi, 10043 Turin, Italy
Department of Neuroscience ‘Rita Levi Montalcini’, University of Turin, 10126 Turin, Italy
Department of Molecular Biotechnology and Health Sciences, University of Turin, 10126 Turin, Italy
Authors to whom correspondence should be addressed.
Academic Editor: Felice Giangaspero
Received: 18 January 2021 / Revised: 17 February 2021 / Accepted: 24 February 2021 / Published: 1 March 2021
Medulloblastoma (MB) is the most frequent brain tumor in children. The standard treatment consists in surgery, followed by radiotherapy and chemotherapy. These therapies are only partially effective, since many patients still die and those who survive suffer from neurological and endocrine disorders. Therefore, more effective therapies are needed. CENPE is a gene critical for normal proliferation and survival of neural progenitors. Since there is evidence that MB cells are very similar to neural progenitors, we hypothesized that CENPE could be an effective target for MB treatment. In MB cell lines, CENPE depletion induced defects in division and resulted in cell death. To consolidate CENPE as a target for MB treatment, we tested GSK923295, a specific inhibitor already in clinical trials for other cancer types. GSK923295 induced effects similar to CENPE depletion at low nM levels, supporting the idea that CENPE’s inhibition could be a viable strategy for MB treatment.