Prognostic Gene Expression, Stemness and Immune Microenvironment in Pediatric Tumors
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. The Prognostic Landscape of Gene Expression
2.2. Immune Cell Infiltration—Composition and Prognostication
2.3. Stemness Correlates with Immature Histology, Clinic-Pathological Features and Survival
2.4. Immune TME and Stemness in WILMS
3. Discussion
4. Materials and Methods
4.1. Gene Expression Data and Study Design
4.2. Assessment of Prognostic Landscape by PRECOG
4.3. Assessment of Immune Infiltration by CIBERSORT
4.4. Calculation of Gene Expression-Based Stemness Index (mRNAsi)
4.5. Tissue Microarray (TMA) Cohort, Immunohistochemistry and Scoring
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Stahl, D.; Knoll, R.; Gentles, A.J.; Vokuhl, C.; Buness, A.; Gütgemann, I. Prognostic Gene Expression, Stemness and Immune Microenvironment in Pediatric Tumors. Cancers 2021, 13, 854. https://doi.org/10.3390/cancers13040854
Stahl D, Knoll R, Gentles AJ, Vokuhl C, Buness A, Gütgemann I. Prognostic Gene Expression, Stemness and Immune Microenvironment in Pediatric Tumors. Cancers. 2021; 13(4):854. https://doi.org/10.3390/cancers13040854
Chicago/Turabian StyleStahl, David, Rainer Knoll, Andrew J. Gentles, Christian Vokuhl, Andreas Buness, and Ines Gütgemann. 2021. "Prognostic Gene Expression, Stemness and Immune Microenvironment in Pediatric Tumors" Cancers 13, no. 4: 854. https://doi.org/10.3390/cancers13040854