Type 2 Innate Lymphoid Cells Protect against Colorectal Cancer Progression and Predict Improved Patient Survival
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. ILC Are Present in a Murine Model of Colon Cancer and Implicated in Tumour Growth
2.2. ILCs Are Increased in Inflammation-Associated Colon Cancer
2.3. The ILC2-Derived Cytokines, IL-5 and IL-13, Are Elevated in CAC
2.4. Loss of ILC2 Results in Increased Tumour Development in CAC
2.5. An ILC2 Gene Signature Is Associated with Increased Overall Survival in CRC
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Cell Culture and Xenograft Establishment
4.3. Isolation of Lymphoid Cells
4.4. Flow Cytometry
4.5. Colitis-Associated Colon Cancer
4.6. Histology
4.7. Training an Intestinal ILC2 Classifier
4.8. Quantification of Infiltrating ILC2 in the TCGA COAD Cohort Using the Intestinal ILC2 Classifier
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Huang, Q.; Jacquelot, N.; Preaudet, A.; Hediyeh-zadeh, S.; Souza-Fonseca-Guimaraes, F.; McKenzie, A.N.J.; Hansbro, P.M.; Davis, M.J.; Mielke, L.A.; Putoczki, T.L.; et al. Type 2 Innate Lymphoid Cells Protect against Colorectal Cancer Progression and Predict Improved Patient Survival. Cancers 2021, 13, 559. https://doi.org/10.3390/cancers13030559
Huang Q, Jacquelot N, Preaudet A, Hediyeh-zadeh S, Souza-Fonseca-Guimaraes F, McKenzie ANJ, Hansbro PM, Davis MJ, Mielke LA, Putoczki TL, et al. Type 2 Innate Lymphoid Cells Protect against Colorectal Cancer Progression and Predict Improved Patient Survival. Cancers. 2021; 13(3):559. https://doi.org/10.3390/cancers13030559
Chicago/Turabian StyleHuang, Qiutong, Nicolas Jacquelot, Adele Preaudet, Soroor Hediyeh-zadeh, Fernando Souza-Fonseca-Guimaraes, Andrew N. J. McKenzie, Philip M. Hansbro, Melissa J. Davis, Lisa A. Mielke, Tracy L. Putoczki, and et al. 2021. "Type 2 Innate Lymphoid Cells Protect against Colorectal Cancer Progression and Predict Improved Patient Survival" Cancers 13, no. 3: 559. https://doi.org/10.3390/cancers13030559