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Article

Landscape of 4D Cell Interaction in Hodgkin and Non-Hodgkin Lymphomas

1
Dr. Senckenberg Institute of Pathology, Goethe University Frankfurt am Main, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany
2
Frankfurt Institute of Advanced Studies, 60438 Frankfurt am Main, Germany
3
Molecular Bioinformatics, Institute of Computer Science, Goethe University Frankfurt am Main, Robert-Mayer-Straße 11-15, 60325 Frankfurt am Main, Germany
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Department of Otolaryngology, Head and Neck Surgery, University Hospital Frankfurt, 60590 Frankfurt am Main, Germany
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Institut Cochin, INSERM U1016/CNRS UMR 8104, Université de Paris, 75014 Paris, France
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Internal Medicine I, Saarland University Medical School, 66421 Homburg, Germany
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Department of Internal Medicine 2, Goethe University Hospital, 60590 Frankfurt am Main, Germany
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Department of Otorhinolaryngology, Head and Neck Surgery, Saarland University Medical Center, 66421 Homburg, Germany
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Comprehensive Cancer Center Ulm (CCCU), University Hospital Ulm, 89070 Ulm, Germany
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Institute of Pathology, Saarland University Medical School, 66421 Homburg, Germany
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José Carreras Center for Immuno- and Gene Therapy, Saarland University Medical School, 66421 Homburg, Germany
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Institute of General Pharmacology and Toxicology, Goethe University Frankfurt am Main, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany
*
Author to whom correspondence should be addressed.
Equally contributed.
Academic Editor: Elisabetta Abruzzese
Cancers 2021, 13(20), 5208; https://doi.org/10.3390/cancers13205208
Received: 14 September 2021 / Revised: 11 October 2021 / Accepted: 12 October 2021 / Published: 17 October 2021
(This article belongs to the Special Issue Hodgkin Lymphoma)
Little is known about the motility and interaction of primary human lymphoma cells in lymph nodes. The aim of this study therefore was to analyze for the first time if there are differences in motility and interaction with bystander cells between different lymphoma types and normal lymph nodes. We observed systematic differences between B cells and PD1-positive T cells. Furthermore, most cases of Hodgkin lymphomas had fast moving PD1-positive T cells, whereas there was little movement in other lymphoma types. Some lymphomas, particularly Hodgkin lymphomas, presented enhanced cell contacts between neoplastic and reactive cells, suggesting a dependency of lymphoma growth on cellular interaction.
Profound knowledge exists about the clinical, morphologic, genomic, and transcriptomic characteristics of most lymphoma entities. However, information is currently lacking on the dynamic behavior of malignant lymphomas. This pilot study aimed to gain insight into the motility of malignant lymphomas and bystander cells in 20 human lymph nodes. Generally, B cells were faster under reactive conditions compared with B cells in malignant lymphomas. In contrast, PD1-positive T cells did not show systematic differences in velocity between reactive and neoplastic conditions in general. However, lymphomas could be divided into two groups: one with fast PD1-positive T cells (e.g., Hodgkin lymphoma and mantle cell lymphoma; means 8.4 and 7.8 µm/min) and another with slower PD1-positive T cells (e.g., mediastinal grey zone lymphoma; mean 3.5 µm/min). Although the number of contacts between lymphoma cells and PD1-positive T cells was similar in different lymphoma types, important differences were observed in the duration of these contacts. Among the lymphomas with fast PD1-positive T cells, contacts were particularly short in mantle cell lymphoma (mean 54 s), whereas nodular lymphocyte-predominant Hodgkin lymphoma presented prolonged contact times (mean 6.1 min). Short contact times in mantle cell lymphoma were associated with the largest spatial displacement of PD1-positive cells (mean 12.3 µm). Although PD1-positive T cells in nodular lymphocyte-predominant Hodgkin lymphoma were fast, they remained in close contact with the lymphoma cells, in line with a dynamic immunological synapse. This pilot study shows for the first time systematic differences in the dynamic behavior of lymphoma and bystander cells between different lymphoma types. View Full-Text
Keywords: migration; cell motility; cell contacts; Hodgkin lymphoma; non-Hodgkin lymphoma migration; cell motility; cell contacts; Hodgkin lymphoma; non-Hodgkin lymphoma
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Figure 1

MDPI and ACS Style

Hartmann, S.; Scharf, S.; Steiner, Y.; Loth, A.G.; Donnadieu, E.; Flinner, N.; Poeschel, V.; Angel, S.; Bewarder, M.; Bein, J.; Brunnberg, U.; Bozzato, A.; Schick, B.; Stilgenbauer, S.; Bohle, R.M.; Thurner, L.; Hansmann, M.-L. Landscape of 4D Cell Interaction in Hodgkin and Non-Hodgkin Lymphomas. Cancers 2021, 13, 5208. https://doi.org/10.3390/cancers13205208

AMA Style

Hartmann S, Scharf S, Steiner Y, Loth AG, Donnadieu E, Flinner N, Poeschel V, Angel S, Bewarder M, Bein J, Brunnberg U, Bozzato A, Schick B, Stilgenbauer S, Bohle RM, Thurner L, Hansmann M-L. Landscape of 4D Cell Interaction in Hodgkin and Non-Hodgkin Lymphomas. Cancers. 2021; 13(20):5208. https://doi.org/10.3390/cancers13205208

Chicago/Turabian Style

Hartmann, Sylvia, Sonja Scharf, Yvonne Steiner, Andreas G. Loth, Emmanuel Donnadieu, Nadine Flinner, Viola Poeschel, Stephanie Angel, Moritz Bewarder, Julia Bein, Uta Brunnberg, Alessandro Bozzato, Bernhard Schick, Stephan Stilgenbauer, Rainer M. Bohle, Lorenz Thurner, and Martin-Leo Hansmann. 2021. "Landscape of 4D Cell Interaction in Hodgkin and Non-Hodgkin Lymphomas" Cancers 13, no. 20: 5208. https://doi.org/10.3390/cancers13205208

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