A Comparative Endocrine Trans-Differentiation Approach to Pancreatic Ductal Adenocarcinoma Cells with Different EMT Phenotypes Identifies Quasi-Mesenchymal Tumor Cells as Those with Highest Plasticity
Abstract
:Simple Summary
Abstract
1. Introduction
2. Material and Methods
2.1. Pancreatic Cell Lines and Primary Culture
2.2. Quantitative Real-Time PCR
2.3. Immunoblotting
2.4. Trans-Differentiation of PDAC-Derived Cells into Pancreatic Endocrine or Insulin-Expressing Cells
2.5. Real-Time Cell Invasion Assay
2.6. Statistics
3. Results
3.1. Epithelial/Mesenchymal Phenotyping of PDAC-Derived Cell Lines Based on ECAD and VIM Expression
3.2. Epithelial/Mesenchymal Phenotyping of the Paired Isogenic COLO 357 and L3.6pl Cell Lines
3.3. The EMT Phenotype of PDAC Cells Determines Their Trans-Differentiation Potential to Pancreatic Endocrine Precursors
3.4. The EMT Phenotype of PDAC Cells Determines Their Trans-Differentiation Potential to Insulin-Expressing Cells
3.5. The Increased Metastatic Potential of L3.6pl Cells Compared with COLO 357 Cells Is Associated with a Higher Trans-Differentiation Potential to Insulin-Expressing Cells
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Schmidtlein, P.M.; Volz, C.; Braun, R.; Thürling, I.; Lapshyna, O.; Wellner, U.F.; Konukiewitz, B.; Lehnert, H.; Marquardt, J.-U.; Ungefroren, H. A Comparative Endocrine Trans-Differentiation Approach to Pancreatic Ductal Adenocarcinoma Cells with Different EMT Phenotypes Identifies Quasi-Mesenchymal Tumor Cells as Those with Highest Plasticity. Cancers 2021, 13, 4663. https://doi.org/10.3390/cancers13184663
Schmidtlein PM, Volz C, Braun R, Thürling I, Lapshyna O, Wellner UF, Konukiewitz B, Lehnert H, Marquardt J-U, Ungefroren H. A Comparative Endocrine Trans-Differentiation Approach to Pancreatic Ductal Adenocarcinoma Cells with Different EMT Phenotypes Identifies Quasi-Mesenchymal Tumor Cells as Those with Highest Plasticity. Cancers. 2021; 13(18):4663. https://doi.org/10.3390/cancers13184663
Chicago/Turabian StyleSchmidtlein, Paula M., Clara Volz, Rüdiger Braun, Isabel Thürling, Olha Lapshyna, Ulrich F. Wellner, Björn Konukiewitz, Hendrik Lehnert, Jens-Uwe Marquardt, and Hendrik Ungefroren. 2021. "A Comparative Endocrine Trans-Differentiation Approach to Pancreatic Ductal Adenocarcinoma Cells with Different EMT Phenotypes Identifies Quasi-Mesenchymal Tumor Cells as Those with Highest Plasticity" Cancers 13, no. 18: 4663. https://doi.org/10.3390/cancers13184663