Loss of CENP-I Impairs Homologous Recombination and Sensitizes Cells to PARP1 Inhibition
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. CENP-I Expression Is Elevated Gliomas as Compared to Normal Brain
2.2. Loss of CENP-I Sensitizes Cells to Genotoxic Stress
2.3. Loss of CENP-I Results in Increased 53BP1 Foci and Delayed Repair Kinetics
2.4. Loss of CENP-I Leads to Increased Chromosomal Aberrations
2.5. Loss of CENP-I Inhibits HR, But Is Dispensable for NHEJ DSB Repair
2.6. Loss of CENP-I Results in Increased R-Loop Formation
3. Discussion
3.1. Involvement of CENP-I and Homologous Recombination Repair
3.2. Therapeutic Potential of CENP-I Targeting
4. Materials and Methods
- siCENPI-01: CAAACCAUUUCGUGUGAGA
- siCENPI-02: CCAUGAACUCUGUGUCUAA
- siCENPI-03: GAAUUAAAUCACUAUUGA
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Dang, T.T.; Morales, J.C. Loss of CENP-I Impairs Homologous Recombination and Sensitizes Cells to PARP1 Inhibition. Cancers 2021, 13, 3202. https://doi.org/10.3390/cancers13133202
Dang TT, Morales JC. Loss of CENP-I Impairs Homologous Recombination and Sensitizes Cells to PARP1 Inhibition. Cancers. 2021; 13(13):3202. https://doi.org/10.3390/cancers13133202
Chicago/Turabian StyleDang, Tuyen T., and Julio C. Morales. 2021. "Loss of CENP-I Impairs Homologous Recombination and Sensitizes Cells to PARP1 Inhibition" Cancers 13, no. 13: 3202. https://doi.org/10.3390/cancers13133202