The Potential of MLN3651 in Combination with Selumetinib as a Treatment for Merlin-Deficient Meningioma
Abstract
:1. Introduction
2. Results
2.1. CRL4-DCAF1 and KSR1 Are Overexpressed in Meningioma Compared to Normal Cells
2.2. Inhibition of CRL4-DCAF1 Neddylation by MLN3651 Induces Apoptosis and Inhibits Proliferation to Reduce Cell Viability in Meningioma
2.3. MLN3651 and DCAF1 Knockdown Effect on the Raf/MEK/ERK Pathway in Meningioma
2.4. Simultaneous Inhibition of CRL4-DCAF1 and MEK1/2 Leads to an Enhanced Therapeutic Response in Meningioma
3. Discussion
4. Materials and Methods
4.1. Human Tissue
4.2. Co-Immunoprecipitation
4.3. Drug Treatments
4.4. Immunocytochemistry
4.5. Immunohistochemistry
4.6. Lentivirus Production and Infection
4.7. Western Blotting
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Lyons Rimmer, J.; Ercolano, E.; Baiz, D.; Makhija, M.; Berger, A.; Sells, T.; Stroud, S.; Hilton, D.; Adams, C.L.; Hanemann, C.O. The Potential of MLN3651 in Combination with Selumetinib as a Treatment for Merlin-Deficient Meningioma. Cancers 2020, 12, 1744. https://doi.org/10.3390/cancers12071744
Lyons Rimmer J, Ercolano E, Baiz D, Makhija M, Berger A, Sells T, Stroud S, Hilton D, Adams CL, Hanemann CO. The Potential of MLN3651 in Combination with Selumetinib as a Treatment for Merlin-Deficient Meningioma. Cancers. 2020; 12(7):1744. https://doi.org/10.3390/cancers12071744
Chicago/Turabian StyleLyons Rimmer, Jade, Emanuela Ercolano, Daniele Baiz, Mahindra Makhija, Allison Berger, Todd Sells, Steve Stroud, David Hilton, Claire L. Adams, and C Oliver Hanemann. 2020. "The Potential of MLN3651 in Combination with Selumetinib as a Treatment for Merlin-Deficient Meningioma" Cancers 12, no. 7: 1744. https://doi.org/10.3390/cancers12071744