Next Article in Journal
Monoclonal Gammopathies of Renal Significance: Renal Biopsy and Beyond
Previous Article in Journal
CXCR4 Inhibition Enhances Efficacy of FLT3 Inhibitors in FLT3-Mutated AML Augmented by Suppressed TGF-β Signaling
Open AccessArticle

Plasma Level of Circular RNA hsa_circ_0000190 Correlates with Tumor Progression and Poor Treatment Response in Advanced Lung Cancers

1
Department of Chest Medicine, Taipei Veterans General Hospital, Taipei 112, Taiwan
2
School of Medicine, National Yang-Ming University, Taipei 112, Taiwan
3
Institute of Clinical Medicine, National Yang-Ming University, Taipei 112, Taiwan
4
Department of Medical Research, Taipei Veterans General Hospital, Taipei 112, Taiwan
5
School of Pharmaceutical Sciences, National Yang-Ming University, Taipei 112, Taiwan
6
Institute of Pharmacology, National Yang-Ming University, Taipei 112, Taiwan
7
Taiwan International Graduate Program in Molecular Medicine, National Yang-Ming University and Academia Sinica, Taipei 112, Taiwan
8
Division of Thoracic Surgery, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan
9
Taipei Cancer Center, Taipei Medical University, Taipei 110, Taiwan
10
Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, New Taipei City 235, Taiwan
11
Division of Pulmonary Medicine, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan
12
Division of Thoracic Surgery, Department of Surgery, MacKay Memorial Hospital, Taipei 104, Taiwan
13
Department of Medicine, MacKay Medical College, Taipei 104, Taiwan
14
Division of Thoracic Surgery, Department of Surgery, Far-Eastern Memorial Hospital, New Taipei City 220, Taiwan
15
Department of Obstetrics and Gynecology, Tri-service General Hospital, National Defense Medical Center, Taipei 114, Taiwan
16
Department of Neurosurgery, Neurological institute, Taipei Veterans General Hospital, Taipei 112, Taiwan
17
Institute of Food Safety and Health Risk Assessment, National Yang Ming University, Taipei 112, Taiwan
*
Author to whom correspondence should be addressed.
Equal contributors.
Cancers 2020, 12(7), 1740; https://doi.org/10.3390/cancers12071740
Received: 26 May 2020 / Revised: 18 June 2020 / Accepted: 22 June 2020 / Published: 30 June 2020
(This article belongs to the Section Cancer Biomarkers)
Lung cancer (LC) causes the majority of cancer-related deaths. Circular RNAs (circRNAs) were reported to play roles in cancers by targeting pro- and anti-oncogenic miRNAs. However, the mechanisms of circRNAs in LC progression and their prognostic value of treatment response remain unclear. By using next generation sequencing (NGS) of LC cell lines’ transcriptomes, we identified highly overexpressed hsa_circ_0000190 and hsa_circ_000164 as potential biomarkers. By using the highly sensitive RT-ddPCR method, these circRNAs were shown to be secreted by cell lines and were detected in human blood. Clinical validation by RT-ddPCR was carried out on 272 (231 LC patients and 41 controls) blood samples. Higher hsa_circ_0000190 levels were associated with larger tumor size (p < 0.0001), worse histological type of adenocarcinoma (p = 0.0028), later stage (p < 0.0001), more distant metastatic organs (p = 0.0039), extrathoracic metastasis (p = 0.0004), and poor survival (p = 0.047) and prognosis. Using liquid biopsy-based RT-ddPCR, we discovered the correlation between increased hsa_circ_0000190 plasma level (p < 0.0001) and higher programmed death-ligand 1 (PD-L1) level in tumor (p = 0.0283). Notably, long-term follow-up of the immunotherapy treated cases showed that upregulated plasma hsa_circ_0000190 level correlated with poor response to systemic therapy and immunotherapy (p = 0.0002, 0.0058, respectively). Secretory circRNAs are detectable in blood by LB-based RT-ddPCR and may serve as blood-based biomarkers to monitor disease progression and treatment efficacy. View Full-Text
Keywords: lung cancer; circular RNA; liquid biopsy; droplet digital PCR; programmed death-ligand 1 lung cancer; circular RNA; liquid biopsy; droplet digital PCR; programmed death-ligand 1
Show Figures

Graphical abstract

MDPI and ACS Style

Luo, Y.-H.; Yang, Y.-P.; Chien, C.-S.; Yarmishyn, A.A.; Ishola, A.A.; Chien, Y.; Chen, Y.-M.; Huang, T.-W.; Lee, K.-Y.; Huang, W.-C.; Tsai, P.-H.; Lin, T.-W.; Chiou, S.-H.; Liu, C.-Y.; Chang, C.-C.; Chen, M.-T.; Wang, M.-L. Plasma Level of Circular RNA hsa_circ_0000190 Correlates with Tumor Progression and Poor Treatment Response in Advanced Lung Cancers. Cancers 2020, 12, 1740.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop