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Article

The Extracellular Matrix Influences Ovarian Carcinoma Cells’ Sensitivity to Cisplatinum: A First Step towards Personalized Medicine

1
Department of Life Sciences, University of Trieste, 34127 Trieste, Italy
2
Institute for Maternal and Child Health, IRCCS Burlo Garofolo, 34134 Trieste, Italy
3
Department of Medical, Surgical and Health Science, University of Trieste, 34129 Trieste, Italy
4
Centro Sociale Oncologico, OSARF, Azienda Sanitaria Universitaria Giuliano Isontina, 34127 Trieste, Italy
*
Author to whom correspondence should be addressed.
Cancers 2020, 12(5), 1175; https://doi.org/10.3390/cancers12051175
Received: 3 March 2020 / Revised: 29 April 2020 / Accepted: 5 May 2020 / Published: 7 May 2020
(This article belongs to the Special Issue Preclinical and Clinical Advances in Ovarian Cancer)
The development of personalized therapies for ovarian carcinoma patients is still hampered by several limitations, mainly the difficulty of predicting patients’ responses to chemotherapy in tumor cells isolated from peritoneal fluids. The main reason for the low predictive power of in vitro assays is related to the modification of the cancer cells’ phenotype induced by the culture conditions, which results in changes to the activation state and drug sensitivity of tumor cells compared to their in vivo properties. We have defined the optimal culture conditions to set up a prognostic test to predict high-grade serous ovarian carcinoma (HGSOC) patients’ responses to platinum chemotherapy. We evaluated the effects of hyaluronic acid (HA) and fibronectin matrices and the contribution of freezing/thawing processes to the cell response to platinum-based treatment, collecting spheroids from the ascitic fluids of 13 patients with stage II or III HGSOC. Our findings indicated that an efficient model used to generate predictive data for in vivo sensitivity to platinum is culturing fresh spheroids on HA, avoiding the use of previously frozen primary tumor cells. The establishment of this easy, reproducible and standardized testing method can significantly contribute to an improvement in therapeutic effectiveness, thus bringing the prospect of personalized therapy closer for ovarian carcinoma patients. View Full-Text
Keywords: ovarian cancer; spheroids; chemoresistance; hyaluronic acid; fibronectin; personalized medicine; HGSOC ovarian cancer; spheroids; chemoresistance; hyaluronic acid; fibronectin; personalized medicine; HGSOC
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MDPI and ACS Style

Balduit, A.; Agostinis, C.; Mangogna, A.; Maggi, V.; Zito, G.; Romano, F.; Romano, A.; Ceccherini, R.; Grassi, G.; Bonin, S.; Bonazza, D.; Zanconati, F.; Ricci, G.; Bulla, R. The Extracellular Matrix Influences Ovarian Carcinoma Cells’ Sensitivity to Cisplatinum: A First Step towards Personalized Medicine. Cancers 2020, 12, 1175. https://doi.org/10.3390/cancers12051175

AMA Style

Balduit A, Agostinis C, Mangogna A, Maggi V, Zito G, Romano F, Romano A, Ceccherini R, Grassi G, Bonin S, Bonazza D, Zanconati F, Ricci G, Bulla R. The Extracellular Matrix Influences Ovarian Carcinoma Cells’ Sensitivity to Cisplatinum: A First Step towards Personalized Medicine. Cancers. 2020; 12(5):1175. https://doi.org/10.3390/cancers12051175

Chicago/Turabian Style

Balduit, Andrea, Chiara Agostinis, Alessandro Mangogna, Veronica Maggi, Gabriella Zito, Federico Romano, Andrea Romano, Rita Ceccherini, Gabriele Grassi, Serena Bonin, Deborah Bonazza, Fabrizio Zanconati, Giuseppe Ricci, and Roberta Bulla. 2020. "The Extracellular Matrix Influences Ovarian Carcinoma Cells’ Sensitivity to Cisplatinum: A First Step towards Personalized Medicine" Cancers 12, no. 5: 1175. https://doi.org/10.3390/cancers12051175

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