Circulating Exosomal MicroRNA-1307-5p as a Predictor for Metastasis in Patients with Hepatocellular Carcinoma
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. Confirmation of Isolated Circulating Exosomes and Identification of Overexpressed Exo-miRs in Metastatic HCC Patients
2.2. Integrative Analyses of Public Gene Expression Datasets to Select Potential Candidate Pro-Metastatic miRs
2.3. Validation of the Clinical Implications of Candidate Exo-miRs in the Validation Cohort
2.4. In Silico Prediction of the Target Genes of miR-1307-5p
3. Discussion
4. Materials and Methods
4.1. Patients and Sample Collection
4.2. Analysis of Gene Expression Omnibus (GEO) Database and TCGA_LIHC
4.3. Cell Culture and AS-miR-1307-5p Transfection
4.4. Blood Exosome Isolation and Total Exosomal RNA Extraction
4.5. Small RNA Sequencing
4.6. TEM
4.7. NTA
4.8. Western Blotting
4.9. Quantitative Reverse-Transcription Polymerase Chain Reaction (qRT-PCR) Analysis
4.10. Prediction of miR Targets
4.11. IPA
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Variables | Plasma-HCC Cohort (n = 52) | Serum-HCC Cohort (n = 91) | |
---|---|---|---|
HCC (n = 52) | Healthy Control (n = 28) | HCC (n = 73) | |
Age (years), mean ± SD | 54.22 ± 9.98 | 34.96 ± 8.18 | 54.62 ± 9.06 |
Male sex, n (%) | 47 (85.5) | 3 (10.7) | 57 (78.1) |
AST, IU/mL | 72.73 ± 77.36 | 16.82 ± 4.06 | 73.81 ± 92.28 |
ALT, IU/mL | 46.11 ± 32.85 | 14.14 ± 7.77 | 49.48 ± 63.53 |
Platelet, x109/L | 161.59 ± 82.04 | 314± 63.33 | 169.85 ± 85.41 |
AFP (ng/mL), mean ± SD | 8000.26 ± 17,325.08 | 1.65 ± 0.58 | 4394.77 ± 14,600.73 |
Etiology, n HBV/HCV/alcohol/others | – | 65/4/3/1 | |
Albumin (g/L), mean ± SD | 4.07 ± 0.60 | 4.23 ± 0.55 | |
Bilirubin (mg/dL), mean ± SD | 1.22 ± 1.81 | 1.49 ± 3.91 | |
INR, mean ± SD | 1.16 ± 0.14 | 1.18 ± 0.19 | |
Modified UICC stage, n (%) | – | 26 (36)/9 (12)/20 (27)/11 (15.4)/7 (9.6) | |
I/II/III/IVa/IVb | |||
Metastasis, n (%) | 25 (48.1)/27 (51.9) | 7 (9.6)/66 (90.4) | |
Yes/No | |||
Vascular invasion, n (%) | – | 29 (54.7)/24 (45.3) | |
Yes/No |
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Eun, J.W.; Seo, C.W.; Baek, G.O.; Yoon, M.G.; Ahn, H.R.; Son, J.A.; Sung, S.; Kim, D.W.; Kim, S.S.; Cho, H.J.; et al. Circulating Exosomal MicroRNA-1307-5p as a Predictor for Metastasis in Patients with Hepatocellular Carcinoma. Cancers 2020, 12, 3819. https://doi.org/10.3390/cancers12123819
Eun JW, Seo CW, Baek GO, Yoon MG, Ahn HR, Son JA, Sung S, Kim DW, Kim SS, Cho HJ, et al. Circulating Exosomal MicroRNA-1307-5p as a Predictor for Metastasis in Patients with Hepatocellular Carcinoma. Cancers. 2020; 12(12):3819. https://doi.org/10.3390/cancers12123819
Chicago/Turabian StyleEun, Jung Woo, Chul Won Seo, Geum Ok Baek, Moon Gyeong Yoon, Hye Ri Ahn, Ju A. Son, Suna Sung, Do Wan Kim, Soon Sun Kim, Hyo Jung Cho, and et al. 2020. "Circulating Exosomal MicroRNA-1307-5p as a Predictor for Metastasis in Patients with Hepatocellular Carcinoma" Cancers 12, no. 12: 3819. https://doi.org/10.3390/cancers12123819