Comprehensive Analysis of SWI/SNF Inactivation in Lung Adenocarcinoma Cell Models
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. More than 75% of LUAD Cell Lines Have a Mutated SWI/SNF Subunit
2.2. mRNA Profiles of LUAD Cell Lines Show a Transcriptional De-Regulation of the SWI/SNF Complex
2.3. Genetic and Epigenetic Factors Contribute to the Protein Loss of the ATPases and ARID Subunits
2.4. ATPases and ARID Protein Expression Profiles Define Four LUAD Cell Line Subgroups
3. Discussion
4. Materials and Methods
4.1. Lung Adenocarcinoma Cell Lines
4.2. DNA and RNA Extraction
4.3. Gene Capture and Targeted Sequencing
4.4. Deep Sequencing Data Analysis
4.5. In Silico Analysis of the SWI/SNF Complex in Lung Adenocarcinoma Cell Lines
4.6. Evaluation of the Functional Impact of Missense Mutations
4.7. Real-Time Quantitative Polymerase Chain Reaction
4.8. Western Blot
4.9. TCGA Data Download
4.10. Statistical Analysis
4.11. Data availability
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Peinado, P.; Andrades, A.; Cuadros, M.; Rodriguez, M.I.; Coira, I.F.; Garcia, D.J.; Álvarez-Perez, J.C.; Baliñas-Gavira, C.; Arenas, A.M.; Patiño-Mercau, J.R.; et al. Comprehensive Analysis of SWI/SNF Inactivation in Lung Adenocarcinoma Cell Models. Cancers 2020, 12, 3712. https://doi.org/10.3390/cancers12123712
Peinado P, Andrades A, Cuadros M, Rodriguez MI, Coira IF, Garcia DJ, Álvarez-Perez JC, Baliñas-Gavira C, Arenas AM, Patiño-Mercau JR, et al. Comprehensive Analysis of SWI/SNF Inactivation in Lung Adenocarcinoma Cell Models. Cancers. 2020; 12(12):3712. https://doi.org/10.3390/cancers12123712
Chicago/Turabian StylePeinado, Paola, Alvaro Andrades, Marta Cuadros, Maria Isabel Rodriguez, Isabel F. Coira, Daniel J. Garcia, Juan Carlos Álvarez-Perez, Carlos Baliñas-Gavira, Alberto M. Arenas, Juan Rodrigo Patiño-Mercau, and et al. 2020. "Comprehensive Analysis of SWI/SNF Inactivation in Lung Adenocarcinoma Cell Models" Cancers 12, no. 12: 3712. https://doi.org/10.3390/cancers12123712