Physical Plasma-Treated Skin Cancer Cells Amplify Tumor Cytotoxicity of Human Natural Killer (NK) Cells
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. Plasma Treatment Inactivated Skin Cancer Cells and Modulated NK-Cell Ligand-Receptor Expression
2.2. Plasma-Treated Tumor Cells Augmented NK-Cell-Mediated Toxicity
2.3. Plasma-Treated Tumor Cells Stimulated the Secretion of Inflammatory Mediators upon Co-Culture with NK-Cells
2.4. H2O2 Treatment Did Not Replicate Results Observed with Plasma Treatment
3. Discussion
4. Experimental Section
4.1. Cell Culture and NK-Cell Isolation
4.2. Plasma Jet Treatment
4.3. Metabolic Activity
4.4. Flow Cytometry
4.5. High Content Imaging
4.6. H2O2 Measurements
4.7. Cytokine Measurement
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Ligand | Fluorochrome | Clone | Supplier |
---|---|---|---|
MIC A,B | APC | 6D4 | BioLegend |
HLA-A,B,C | PE-Cy7 | G46-2.6 | BD Biosciences |
HLA-E | PE | 3D12 | BioLegend |
PD-L1 | PerCP/Cyanine5.5 | 29E2A3 | BioLegend |
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Clemen, R.; Heirman, P.; Lin, A.; Bogaerts, A.; Bekeschus, S. Physical Plasma-Treated Skin Cancer Cells Amplify Tumor Cytotoxicity of Human Natural Killer (NK) Cells. Cancers 2020, 12, 3575. https://doi.org/10.3390/cancers12123575
Clemen R, Heirman P, Lin A, Bogaerts A, Bekeschus S. Physical Plasma-Treated Skin Cancer Cells Amplify Tumor Cytotoxicity of Human Natural Killer (NK) Cells. Cancers. 2020; 12(12):3575. https://doi.org/10.3390/cancers12123575
Chicago/Turabian StyleClemen, Ramona, Pepijn Heirman, Abraham Lin, Annemie Bogaerts, and Sander Bekeschus. 2020. "Physical Plasma-Treated Skin Cancer Cells Amplify Tumor Cytotoxicity of Human Natural Killer (NK) Cells" Cancers 12, no. 12: 3575. https://doi.org/10.3390/cancers12123575