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Article

MiR-378a-3p Is Critical for Burkitt Lymphoma Cell Growth

1
Departments of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, 9700 RB Groningen, The Netherlands
2
Institute of Human Genetics, Polish Academy of Sciences, 60-479 Poznan, Poland
3
Biotechnology Centre, Silesian University of Technology, 44-100 Gliwice, Poland
4
Department of Genetics, University Medical Center Groningen, University of Groningen, 9700 RB Groningen, The Netherlands
*
Author to whom correspondence should be addressed.
Cancers 2020, 12(12), 3546; https://doi.org/10.3390/cancers12123546
Received: 16 October 2020 / Revised: 9 November 2020 / Accepted: 25 November 2020 / Published: 27 November 2020
(This article belongs to the Special Issue Role of miRNAs in Cancer—Analysis of Their Targetome)
MicroRNAs (miRNAs) are small RNAs that regulate expression of specific target genes. We observed elevated levels of miR-378a-3p in Burkitt lymphoma (BL) and studied its role in the pathogenesis of BL. Inhibition of miR-378a-3p reduced growth of BL cells, confirming its significance in BL. Identification of BL specific target genes of miR-378a-3p revealed four candidates. For two of them, MNT and IRAK4, miR-378a-dependent regulation was confirmed at the protein level. Overexpression of MNT and IRAK4 in BL cell lines resulted in a similar effect as observed upon miR-378a-3p inhibition, suggesting their involvement in the growth regulatory role of miR-378a-3p.
MicroRNAs (miRNAs) are small RNA molecules with important gene regulatory roles in normal and pathophysiological cellular processes. Burkitt lymphoma (BL) is an MYC-driven lymphoma of germinal center B (GC-B) cell origin. To gain further knowledge on the role of miRNAs in the pathogenesis of BL, we performed small RNA sequencing in BL cell lines and normal GC-B cells. This revealed 26 miRNAs with significantly different expression levels. For five miRNAs, the differential expression pattern was confirmed in primary BL tissues compared to GC-B cells. MiR-378a-3p was upregulated in BL, and its inhibition reduced the growth of multiple BL cell lines. RNA immunoprecipitation of Argonaute 2 followed by microarray analysis (Ago2-RIP-Chip) upon inhibition and ectopic overexpression of miR-378a-3p revealed 63 and 20 putative miR-378a-3p targets, respectively. Effective targeting by miR-378a-3p was confirmed by luciferase reporter assays for MAX Network Transcriptional Repressor (MNT), Forkhead Box P1 (FOXP1), Interleukin 1 Receptor Associated Kinase 4 (IRAK4), and lncRNA Just Proximal To XIST (JPX), and by Western blot for IRAK4 and MNT. Overexpression of IRAK4 and MNT phenocopied the effect of miR-378a-3p inhibition. In summary, we identified miR-378a-3p as a miRNA with an oncogenic role in BL and identified IRAK4 and MNT as miR-378a-3p target genes that are involved in its growth regulatory role. View Full-Text
Keywords: Burkitt lymphoma; miR-378a-3p; cell growth; microRNA Burkitt lymphoma; miR-378a-3p; cell growth; microRNA
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MDPI and ACS Style

Niu, F.; Dzikiewicz-Krawczyk, A.; Koerts, J.; de Jong, D.; Wijenberg, L.; Fernandez Hernandez, M.; Slezak-Prochazka, I.; Winkle, M.; Kooistra, W.; van der Sluis, T.; Rutgers, B.; Terpstra, M.M.; Kok, K.; Kluiver, J.; van den Berg, A. MiR-378a-3p Is Critical for Burkitt Lymphoma Cell Growth. Cancers 2020, 12, 3546. https://doi.org/10.3390/cancers12123546

AMA Style

Niu F, Dzikiewicz-Krawczyk A, Koerts J, de Jong D, Wijenberg L, Fernandez Hernandez M, Slezak-Prochazka I, Winkle M, Kooistra W, van der Sluis T, Rutgers B, Terpstra MM, Kok K, Kluiver J, van den Berg A. MiR-378a-3p Is Critical for Burkitt Lymphoma Cell Growth. Cancers. 2020; 12(12):3546. https://doi.org/10.3390/cancers12123546

Chicago/Turabian Style

Niu, Fubiao, Agnieszka Dzikiewicz-Krawczyk, Jasper Koerts, Debora de Jong, Laura Wijenberg, Margot Fernandez Hernandez, Izabella Slezak-Prochazka, Melanie Winkle, Wierd Kooistra, Tineke van der Sluis, Bea Rutgers, Miente M. Terpstra, Klaas Kok, Joost Kluiver, and Anke van den Berg. 2020. "MiR-378a-3p Is Critical for Burkitt Lymphoma Cell Growth" Cancers 12, no. 12: 3546. https://doi.org/10.3390/cancers12123546

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