In Silico and In Vitro Analysis of lncRNA XIST Reveals a Panel of Possible Lung Cancer Regulators and a Five-Gene Diagnostic Signature
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. Expression Level of XIST, TSIX, hnRNPu, Bcl-2, and BRCA1 in NSCLC
2.2. Functional Analysis
3. Discussion
4. Materials and Methods
4.1. Cell Lines
4.2. siRNA for XIST
4.3. RNA Isolation, cDNA Synthesis, and qPCR
4.4. RT-qPCR
4.5. RNAseq
4.6. Bioinformatic Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Primer | Sequence 5′–3′ |
---|---|
18S RNA-Forward | ATGGCCGTTCTGAGTTGGTG |
18S RNA-Reverse | CGCTGAGCCAGTCAGTGTAG |
PRKCQ-Forward | CTTGTGGCAGCTTTGGATGT |
PRKCQ-Reverse | CGTTTCTGACGCACATGTTT |
NrCAM-Forward | TTGTGCAAAGAGGGAGCATG |
NrCAM-Reverse | GGGCAGTTCCCTGTTGTCCT |
CDKN1A-Forward | GCAGACCAGCATGACAGATTT |
CDKN1A-Reverse | GGATTAGGGCTTCCTCTTGGA |
RHOH Forward | GAGAAGTAACATTCTGCAAATCGC |
RHOH Reverse | AGCACACGCCATTCAGCAAG |
XIST-Forward | AGGTCAGGCAGAGGAAGTCA |
XIST-Reverse | AGGTCAGGCAGAGGAAGTCA |
ROX1-AS1 Forward | CTAGTTAGCAGGGGCAGCAC |
PROX1-AS1 Reverse | AACAGAGAGGCGTGGAAGAA |
MYC-Forward | TGGACATCCGCAAAGACCTGTAC |
MYC-Reverse | TCAGGAGGAGCAATGATCTTGA |
SFTA1P-Forward | CAGCATTCCAGGTGGGCTTT |
SFTA1P-Reverse | CCTTGTTTGGCTTACTCGTGC |
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Katopodis, P.; Dong, Q.; Halai, H.; Fratila, C.I.; Polychronis, A.; Anikin, V.; Sisu, C.; Karteris, E. In Silico and In Vitro Analysis of lncRNA XIST Reveals a Panel of Possible Lung Cancer Regulators and a Five-Gene Diagnostic Signature. Cancers 2020, 12, 3499. https://doi.org/10.3390/cancers12123499
Katopodis P, Dong Q, Halai H, Fratila CI, Polychronis A, Anikin V, Sisu C, Karteris E. In Silico and In Vitro Analysis of lncRNA XIST Reveals a Panel of Possible Lung Cancer Regulators and a Five-Gene Diagnostic Signature. Cancers. 2020; 12(12):3499. https://doi.org/10.3390/cancers12123499
Chicago/Turabian StyleKatopodis, Periklis, Qiduo Dong, Heerni Halai, Cristian I. Fratila, Andreas Polychronis, Vladimir Anikin, Cristina Sisu, and Emmanouil Karteris. 2020. "In Silico and In Vitro Analysis of lncRNA XIST Reveals a Panel of Possible Lung Cancer Regulators and a Five-Gene Diagnostic Signature" Cancers 12, no. 12: 3499. https://doi.org/10.3390/cancers12123499