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Article

miR-10a-5p and miR-29b-3p as Extracellular Vesicle-Associated Prostate Cancer Detection Markers

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Department of Urology and Urosurgery, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany
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Division of Signaling and Functional Genomics, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany
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Omics IT and Data Management Core Facility (ODCF), German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany
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Genomics and Proteomics Core Facility, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany
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Departments of Haematology and Oncology and Developmental Biochemistry, University Medicine Göttingen, 37075 Göttingen, Germany
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Department of Gastrointestinal, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany
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Institute of Transfusion Medicine and Immunology, FlowCore Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany
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Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany
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Department of Applied Tumor Biology, Institute of Pathology, Heidelberg University Hospital, 69120 Heidelberg, Germany
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Clinical Cooperation Unit Applied Tumor Biology, German Cancer Research Centre (DKFZ), 69120 Heidelberg, Germany
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Institute of Pathology, Medical Faculty Mannheim, Heidelberg Heidelberg, 68167 Mannheim, Germany
*
Author to whom correspondence should be addressed.
Cancers 2020, 12(1), 43; https://doi.org/10.3390/cancers12010043
Received: 3 December 2019 / Revised: 17 December 2019 / Accepted: 18 December 2019 / Published: 21 December 2019
(This article belongs to the Special Issue New Biomarkers in Cancers)
Extracellular vesicles (EVs) are shed by many different cell types. Their nucleic acids content offers new opportunities for biomarker research in different solid tumors. The role of EV RNA in prostate cancer (PCa) is still largely unknown. EVs were isolated from different benign and malignant prostate cell lines and blood plasma from patients with PCa (n = 18) and controls with benign prostatic hyperplasia (BPH) (n = 7). Nanoparticle tracking analysis (NTA), Western blot, electron microscopy, and flow cytometry analysis were used for the characterization of EVs. Non-coding RNA expression profiling of PC3 metastatic PCa cells and their EVs was performed by next generation sequencing (NGS). miRNAs differentially expressed in PC3 EVs were validated with qRT-PCR in EVs derived from additional cell lines and patient plasma and from matched tissue samples. 92 miRNAs were enriched and 48 miRNAs were depleted in PC3 EVs compared to PC3 cells, which could be confirmed by qRT-PCR. miR-99b-5p was significantly higher expressed in malignant compared to benign EVs. Furthermore, expression profiling showed miR-10a-5p (p = 0.018) and miR-29b-3p (p = 0.002), but not miR-99b-5p, to be overexpressed in plasma-derived EVs from patients with PCa compared with controls. In the corresponding tissue samples, no significant differences in the miRNA expression could be observed. We thus propose that EV-associated miR-10a-5p and miR-29b-3p could serve as potential new PCa detection markers. View Full-Text
Keywords: prostate cancer; extracellular vesicles; next generation sequencing; qRT-PCR; non-coding RNA; miRNA; mir-10a-5p; miR-29b-3p; miR-99b-5p prostate cancer; extracellular vesicles; next generation sequencing; qRT-PCR; non-coding RNA; miRNA; mir-10a-5p; miR-29b-3p; miR-99b-5p
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MDPI and ACS Style

Worst, T.S.; Previti, C.; Nitschke, K.; Diessl, N.; Gross, J.C.; Hoffmann, L.; Frey, L.; Thomas, V.; Kahlert, C.; Bieback, K.; Torres Crigna, A.; Fricke, F.; Porubsky, S.; Westhoff, N.; von Hardenberg, J.; Nuhn, P.; Erben, P.; Michel, M.S.; Boutros, M. miR-10a-5p and miR-29b-3p as Extracellular Vesicle-Associated Prostate Cancer Detection Markers. Cancers 2020, 12, 43. https://doi.org/10.3390/cancers12010043

AMA Style

Worst TS, Previti C, Nitschke K, Diessl N, Gross JC, Hoffmann L, Frey L, Thomas V, Kahlert C, Bieback K, Torres Crigna A, Fricke F, Porubsky S, Westhoff N, von Hardenberg J, Nuhn P, Erben P, Michel MS, Boutros M. miR-10a-5p and miR-29b-3p as Extracellular Vesicle-Associated Prostate Cancer Detection Markers. Cancers. 2020; 12(1):43. https://doi.org/10.3390/cancers12010043

Chicago/Turabian Style

Worst, Thomas S., Christopher Previti, Katja Nitschke, Nicolle Diessl, Julia C. Gross, Lena Hoffmann, Lisa Frey, Vanessa Thomas, Christoph Kahlert, Karen Bieback, Adriana Torres Crigna, Fabia Fricke, Stefan Porubsky, Niklas Westhoff, Jost von Hardenberg, Philipp Nuhn, Philipp Erben, Maurice S. Michel, and Michael Boutros. 2020. "miR-10a-5p and miR-29b-3p as Extracellular Vesicle-Associated Prostate Cancer Detection Markers" Cancers 12, no. 1: 43. https://doi.org/10.3390/cancers12010043

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