Loss of Rb1 Enhances Glycolytic Metabolism in Kras-Driven Lung Tumors In Vivo
Abstract
:1. Introduction
2. Results
2.1. Steady-State Metabolomics Highlights Metabolic Discrepancies in Rb1-Deficient Lung Tumors In Vivo
2.2. Loss of Rb1 Enhances Glycolysis in Kras-Driven Lung Tumors
2.3. Rb1 Does Not Regulate Glutamine Utilization In Vivo
2.4. Loss of Rb1 Does Not Alter Lactate Utilization in Kras-Driven Lung Tumors
3. Discussion
4. Materials and Methods
4.1. Mouse Model and Adenoviral Infection
4.2. Kaplan-Meier Analysis
4.3. [U-13C]-Glucose, [U-13C,15N]-Glutamine, and [U-13C]-Lactate Tracer Studies
4.4. Plasma [U-13C]-Glucose, [U-13C,15N]-Glutamine, and [U-13C]-Lactate Analysis
4.5. Metabolite Tissue Sample Preparation for Tracer Studies
4.6. Sample Preparation and Derivatization for Steady-State Metabolomics
4.7. GC-MS Analysis and Data Processing
4.8. 2D-LC-MS/MS Analysis and Data Processing
4.9. Data Analysis for 2D-LC-MS/MS
4.10. 2D-NMR Analysis
4.11. Real Time-PCR
4.12. Immunohistochemistry
4.13. Immunoblotting
4.14. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Conroy, L.R.; Dougherty, S.; Kruer, T.; Metcalf, S.; Lorkiewicz, P.; He, L.; Yin, X.; Zhang, X.; Arumugam, S.; Young, L.E.A.; et al. Loss of Rb1 Enhances Glycolytic Metabolism in Kras-Driven Lung Tumors In Vivo. Cancers 2020, 12, 237. https://doi.org/10.3390/cancers12010237
Conroy LR, Dougherty S, Kruer T, Metcalf S, Lorkiewicz P, He L, Yin X, Zhang X, Arumugam S, Young LEA, et al. Loss of Rb1 Enhances Glycolytic Metabolism in Kras-Driven Lung Tumors In Vivo. Cancers. 2020; 12(1):237. https://doi.org/10.3390/cancers12010237
Chicago/Turabian StyleConroy, Lindsey R., Susan Dougherty, Traci Kruer, Stephanie Metcalf, Pawel Lorkiewicz, Liqing He, Xinmin Yin, Xiang Zhang, Sengodagounder Arumugam, Lyndsay E.A. Young, and et al. 2020. "Loss of Rb1 Enhances Glycolytic Metabolism in Kras-Driven Lung Tumors In Vivo" Cancers 12, no. 1: 237. https://doi.org/10.3390/cancers12010237