Next Article in Journal
Clinical Significance of ABCB1 in Acute Myeloid Leukemia: A Comprehensive Study
Next Article in Special Issue
Hybrid Clustered Nanoparticles for Chemo-Antibacterial Combinatorial Cancer Therapy
Previous Article in Journal
Everything in Moderation: Lessons Learned by Exploiting Moderate Replication Stress in Cancer
Previous Article in Special Issue
Multi-Drug/Gene NASH Therapy Delivery and Selective Hyperspectral NIR Imaging Using Chirality-Sorted Single-Walled Carbon Nanotubes
Open AccessArticle

A Tunable Nanoplatform of Nanogold Functionalised with Angiogenin Peptides for Anti-Angiogenic Therapy of Brain Tumours

1
Consorzio Interuniversitario di Ricerca in Chimica dei Metalli nei Sistemi Biologici (CIRCMSB), Via Celso Ulpiani 27, I-70126 Bari, Italy
2
Hybrid NanobioInterfaces Lab (NHIL), Department of Chemical Sciences, University of Catania, Viale Andrea Doria 6, I-95125 Catania, Italy
3
Department of Biomedical and Biotechnological Sciences, University of Catania, Via Santa Sofia 89, I-95123 Catania, Italy
4
Institute of Crystallography Catania, National Council of Research (IC-CNR), Via Paolo Gaifami 18, I-95126 Catania, Italy
5
Department of Pharmacy, University of Pisa, via Bonanno Pisano 6, I-56126 Pisa, Italy
*
Authors to whom correspondence should be addressed.
Cancers 2019, 11(9), 1322; https://doi.org/10.3390/cancers11091322
Received: 9 August 2019 / Revised: 1 September 2019 / Accepted: 3 September 2019 / Published: 6 September 2019
(This article belongs to the Special Issue Cancer Nanomedicine)
Angiogenin (ANG), an endogenous protein that plays a key role in cell growth and survival, has been scrutinised here as promising nanomedicine tool for the modulation of pro-/anti-angiogenic processes in brain cancer therapy. Specifically, peptide fragments from the putative cell membrane binding domain (residues 60–68) of the protein were used in this study to obtain peptide-functionalised spherical gold nanoparticles (AuNPs) of about 10 nm and 30 nm in optical and hydrodynamic size, respectively. Different hybrid biointerfaces were fabricated by peptide physical adsorption (Ang60–68) or chemisorption (the cysteine analogous Ang60–68Cys) at the metal nanoparticle surface, and cellular assays were performed in the comparison with ANG-functionalised AuNPs. Cellular treatments were performed both in basal and in copper-supplemented cell culture medium, to scrutinise the synergic effect of the metal, which is another known angiogenic factor. Two brain cell lines were investigated in parallel, namely tumour glioblastoma (A172) and neuron-like differentiated neuroblastoma (d-SH-SY5Y). Results on cell viability/proliferation, cytoskeleton actin, angiogenin translocation and vascular endothelial growth factor (VEGF) release pointed to the promising potentialities of the developed systems as anti-angiogenic tunable nanoplaftforms in cancer cells treatment. View Full-Text
Keywords: plasmonics; nanomedicine; theranostics; copper; VEGF; glioblastoma; differentiated neuroblastoma; peptidomimetics; real-time quantitative polymerase chain reaction (qPCR); actin plasmonics; nanomedicine; theranostics; copper; VEGF; glioblastoma; differentiated neuroblastoma; peptidomimetics; real-time quantitative polymerase chain reaction (qPCR); actin
Show Figures

Graphical abstract

MDPI and ACS Style

Naletova, I.; Cucci, L.M.; D’Angeli, F.; Anfuso, C.D.; Magrì, A.; La Mendola, D.; Lupo, G.; Satriano, C. A Tunable Nanoplatform of Nanogold Functionalised with Angiogenin Peptides for Anti-Angiogenic Therapy of Brain Tumours. Cancers 2019, 11, 1322.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop