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New Phosphorylation Sites of Rad51 by c-Met Modulates Presynaptic Filament Stability

1
Group of Mechanism and Regulation of DNA Repair, UFIP UMR CNRS 6286/University of Nantes, 44322 Nantes, France
2
Group of Molecular Engineering and Glycobiology, UFIP UMR CNRS 6286/University of Nantes, 44322 Nantes, France
3
CRCINA, INSERM, CNRS, University of Angers, University of Nantes, 44007 Nantes, France
*
Author to whom correspondence should be addressed.
Cancers 2019, 11(3), 413; https://doi.org/10.3390/cancers11030413
Received: 15 February 2019 / Revised: 10 March 2019 / Accepted: 20 March 2019 / Published: 23 March 2019
(This article belongs to the Special Issue Protein Kinases and Cancers)
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Abstract

Genomic instability through deregulation of DNA repair pathways can initiate cancer and subsequently result in resistance to chemo and radiotherapy. Understanding these biological mechanisms is therefore essential to overcome cancer. RAD51 is the central protein of the Homologous Recombination (HR) DNA repair pathway, which leads to faithful DNA repair of DSBs. The recombinase activity of RAD51 requires nucleofilament formation and is regulated by post-translational modifications such as phosphorylation. In the last decade, studies have suggested the existence of a relationship between receptor tyrosine kinases (RTK) and Homologous Recombination DNA repair. Among these RTK the c-MET receptor is often overexpressed or constitutively activated in many cancer types and its inhibition induces the decrease of HR. In this study, we show for the first time that c-MET is able to phosphorylate the RAD51 protein. We demonstrate in vitro that c-MET phosphorylates four tyrosine residues localized mainly in the subunit-subunit interface of RAD51. Whereas these post-translational modifications do not affect the presynaptic filament formation, they strengthen its stability against the inhibitor effect of the BRC peptide obtained from BRCA2. Taken together, these results confirm the role of these modifications in the regulation of the BRCA2-RAD51 interaction and underline the importance of c-MET in DNA damage response. View Full-Text
Keywords: RAD51 recombinase; HGFR kinase; post-translational modification; DNA repair; cancer outcomes RAD51 recombinase; HGFR kinase; post-translational modification; DNA repair; cancer outcomes
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Chabot, T.; Defontaine, A.; Marquis, D.; Renodon-Corniere, A.; Courtois, E.; Fleury, F.; Cheraud, Y. New Phosphorylation Sites of Rad51 by c-Met Modulates Presynaptic Filament Stability. Cancers 2019, 11, 413.

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