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Article

Imaging Collagen Alterations in STICs and High Grade Ovarian Cancers in the Fallopian Tubes by Second Harmonic Generation Microscopy

1
Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USA
2
Department of Obstetrics and Gynecology, University of Wisconsin, Madison, WI 53706, USA
3
Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia, PA 19104, USA
*
Author to whom correspondence should be addressed.
Cancers 2019, 11(11), 1805; https://doi.org/10.3390/cancers11111805
Received: 1 November 2019 / Accepted: 14 November 2019 / Published: 16 November 2019
(This article belongs to the Special Issue Ovarian Cancer Metastasis)
The majority of high-grade serous ovarian cancers originate in the fallopian tubes, however, the corresponding structural changes in the extracellular matrix (ECM) have not been well-characterized. This information could provide new insight into the carcinogenesis and provide the basis for new diagnostic tools. We have previously used the collagen-specific Second Harmonic Generation (SHG) microscopy to probe collagen fiber alterations in high-grade serous ovarian cancer and in other ovarian tumors, and showed they could be uniquely identified by machine learning approaches. Here we couple SHG imaging of serous tubal intra-epithelial carcinomas (STICs), high-grade cancers, and normal regions of the fallopian tubes, using three distinct image analysis approaches to form a classification scheme based on the respective collagen fiber morphology. Using a linear discriminant analysis, we achieved near 100% classification accuracy between high-grade disease and the other tissues, where the STICs and normal regions were differentiated with ~75% accuracy. Importantly, the collagen in high-grade disease in both the fallopian tube and the ovary itself have a similar collagen morphology, further substantiating the metastasis between these sites. This analysis provides a new method of classification, but also quantifies the structural changes in the disease, which may provide new insight into metastasis. View Full-Text
Keywords: collagen fibers; second harmonic generation; image analysis; STIC; high grade collagen fibers; second harmonic generation; image analysis; STIC; high grade
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MDPI and ACS Style

Rentchler, E.C.; Gant, K.L.; Drapkin, R.; Patankar, M.; J. Campagnola, P. Imaging Collagen Alterations in STICs and High Grade Ovarian Cancers in the Fallopian Tubes by Second Harmonic Generation Microscopy. Cancers 2019, 11, 1805. https://doi.org/10.3390/cancers11111805

AMA Style

Rentchler EC, Gant KL, Drapkin R, Patankar M, J. Campagnola P. Imaging Collagen Alterations in STICs and High Grade Ovarian Cancers in the Fallopian Tubes by Second Harmonic Generation Microscopy. Cancers. 2019; 11(11):1805. https://doi.org/10.3390/cancers11111805

Chicago/Turabian Style

Rentchler, Eric C., Kristal L. Gant, Ronny Drapkin, Manish Patankar, and Paul J. Campagnola. 2019. "Imaging Collagen Alterations in STICs and High Grade Ovarian Cancers in the Fallopian Tubes by Second Harmonic Generation Microscopy" Cancers 11, no. 11: 1805. https://doi.org/10.3390/cancers11111805

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