Antineoplastic Effect of a Combined Mitotane Treatment/Ionizing Radiation in Adrenocortical Carcinoma: A Preclinical Study
Abstract
:1. Introduction
2. Results
2.1. Effect of Treatment on the Volume and Morphology of Tumors in the Xenograft Model
2.2. MRI and Histological Findings Confirm the Inhibitory Effect of the Combined Treatment
2.3. The Treatments Induce Different Effects on Steroidogenesis
2.4. Combined MTT/IR Treatment Interferes with the Cell Cycle and Induces Cell Death in the ACC Xenograft Model
2.5. Role of MSH2 in Cell Cycle Regulation
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Animals
4.3. Mitotane
4.4. Ionizing Radiation
4.5. In vivo Magnetic Resonance Imaging (MRI) Study
4.6. Histopathology
4.7. Tumor Cells and Cell Cycle Analysis
4.8. Steroid Assays
4.9. MSH2 Silencing
4.10. Western Blotting Analysis
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Cerquetti, L.; Bucci, B.; Carpinelli, G.; Lardo, P.; Proietti, A.; Saporito, R.; Rindi, G.; Petrangeli, E.; Toscano, V.; Stigliano, A. Antineoplastic Effect of a Combined Mitotane Treatment/Ionizing Radiation in Adrenocortical Carcinoma: A Preclinical Study. Cancers 2019, 11, 1768. https://doi.org/10.3390/cancers11111768
Cerquetti L, Bucci B, Carpinelli G, Lardo P, Proietti A, Saporito R, Rindi G, Petrangeli E, Toscano V, Stigliano A. Antineoplastic Effect of a Combined Mitotane Treatment/Ionizing Radiation in Adrenocortical Carcinoma: A Preclinical Study. Cancers. 2019; 11(11):1768. https://doi.org/10.3390/cancers11111768
Chicago/Turabian StyleCerquetti, Lidia, Barbara Bucci, Giulia Carpinelli, Pina Lardo, Antonella Proietti, Raffaele Saporito, Guido Rindi, Elisa Petrangeli, Vincenzo Toscano, and Antonio Stigliano. 2019. "Antineoplastic Effect of a Combined Mitotane Treatment/Ionizing Radiation in Adrenocortical Carcinoma: A Preclinical Study" Cancers 11, no. 11: 1768. https://doi.org/10.3390/cancers11111768