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Cancers 2018, 10(8), 276; https://doi.org/10.3390/cancers10080276

Sperm Protein 17 Expression by Murine Epithelial Ovarian Cancer Cells and Its Impact on Tumor Progression

1
Department of Immunology and Pathology, Central Clinical School, Faculty of Medicine, Nursing and Health Science, Monash University, Melbourne, Victoria 3004, Australia
2
Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha 410008, China
3
Centre for Cancer Research, Hudson Institute of Medical Research, Clayton, Victoria 3168, Australia
4
Department of Molecular and Translational Sciences, Monash University, Clayton, Victoria 3168, Australia
5
School of Health and Biomedical Sciences, RMIT University, Bundoora, Victoria 3083, Australia
*
Authors to whom correspondence should be addressed.
Received: 29 June 2018 / Revised: 13 August 2018 / Accepted: 14 August 2018 / Published: 20 August 2018
(This article belongs to the Special Issue Cancer Vaccines: Research and Applications)
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Abstract

The cancer testis antigen sperm protein 17 (Sp17) is a promising antigenic target in epithelial ovarian cancer (EOC) vaccine development. However, its role in ovarian cancer is unclear. We isolated and expanded Sp17+ and Sp17 clones from the murine EOC cell line ID8, and compared their in-vitro cell growth characteristics and in-vivo tumorigenicity. We also examined the potential co-expression of molecules that may influence cancer cell survival and interaction with immune cells. These include stimulatory and immunosuppressive molecules, such as major histocompatibility class I molecules (MHC I), MHC II, cytotoxic T lymphocyte associated antigen-4 (CTLA-4), CD73, CD39, tumor necrosis factor receptor II (TNFRII), signal transducer and activator of transcription 3 (STAT3) and programmed death-ligand 1 (PD-L1). Whilst the presence of Sp17 was not correlated with the ID8 cell proliferation/growth capacity in vitro, it was critical to enable progressive tumor formation in vivo. Flow cytometry revealed that Sp17+ ID8 cells displayed higher expression of both STAT3 and PD-L1, whilst MHC II expression was lower. Moreover, Sp17high (PD-L1+MHCII) cell populations showed significantly enhanced resistance to Paclitaxel-induced cell death in vitro compared to Sp17low (PD-L1MHCII+) cells, which was associated in turn with increased STAT3 expression. Together, the data support Sp17 as a factor associated with in-vivo tumor progression and chemo-resistance, validating it as a suitable target for vaccine development. View Full-Text
Keywords: sperm protein 17 (Sp17); ID8; PD-L1; STAT3; MHC II; Paclitaxel; tumor resistant; immune evasion sperm protein 17 (Sp17); ID8; PD-L1; STAT3; MHC II; Paclitaxel; tumor resistant; immune evasion
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Gao, Q.; Xiang, S.D.; Wilson, K.; Madondo, M.; Stephens, A.N.; Plebanski, M. Sperm Protein 17 Expression by Murine Epithelial Ovarian Cancer Cells and Its Impact on Tumor Progression. Cancers 2018, 10, 276.

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