Recombinant TSR1 of ADAMTS5 Suppresses Melanoma Growth in Mice via an Anti-angiogenic Mechanism
Abstract
:1. Introduction
2. Results
2.1. Recombinant Type 1 Repeat Domain Induces Caspase-Dependent Apoptosis in Human Umbilical Vein Endothelial Cells
2.2. Recombinant Type 1 Repeat Domain Suppressed the B16F10 Melanoma Growth in Mice
2.3. Recombinant Type 1 Repeat Domain Suppresses B16 Melaonma Growth by Inhibiting Tumor Angiogenesis and Tumor Cell Proliferation While Inducing Tumor Cell Apoptosis
3. Discussion
4. Materials and Methods
4.1. Recombinant Type 1 Repeat Domain Production and Purification
4.2. Cell Culture
4.3. Apoptosis Determination by IncuCyte ZOOM Live-Cell Analysis System
4.4. Western Blot
4.5. Immunohistochemistry
4.6. Xenograft Mouse Tumorigenesis Assay
4.7. Mice Serum Isolation
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Renganathan, B.; Durairaj, V.; Kirman, D.C.; Esubonteng, P.K.A.; Ang, S.K.; Ge, R. Recombinant TSR1 of ADAMTS5 Suppresses Melanoma Growth in Mice via an Anti-angiogenic Mechanism. Cancers 2018, 10, 192. https://doi.org/10.3390/cancers10060192
Renganathan B, Durairaj V, Kirman DC, Esubonteng PKA, Ang SK, Ge R. Recombinant TSR1 of ADAMTS5 Suppresses Melanoma Growth in Mice via an Anti-angiogenic Mechanism. Cancers. 2018; 10(6):192. https://doi.org/10.3390/cancers10060192
Chicago/Turabian StyleRenganathan, Bhuvanasundar, Vinoth Durairaj, Dogan Can Kirman, Paa Kow A. Esubonteng, Swee Kim Ang, and Ruowen Ge. 2018. "Recombinant TSR1 of ADAMTS5 Suppresses Melanoma Growth in Mice via an Anti-angiogenic Mechanism" Cancers 10, no. 6: 192. https://doi.org/10.3390/cancers10060192