Towards Multiplex Molecular Diagnosis—A Review of Microfluidic Genomics Technologies
Abstract
:1. Introduction
2. Lysis Techniques
2.1. Chemical Lysis
2.2. Mechanical Lysis
2.3. Electrical Lysis
2.4. Thermal Lysis Techniques
3. Nucleic Acid Extraction
3.1. Solid Phase Extraction
3.2. Isotachophoresis
4. Amplification
4.1. Cyclo-Thermal Amplification
4.1.1. Stationary Chamber Polymerase Chain Reaction (PCR)
4.1.2. Continuous-Flow PCR
4.1.3. Multiple Annealing and Looping-Based Amplification Cycles (MALBAC)
4.2. Isothermal Amplification
4.2.1. Nucleic Acid Sequence-Based Amplification (NASBA)
4.2.2. Loop-Mediated Isothermal Amplification (LAMP)
4.2.3. Recombinase Polymerase Amplification (RPA)
4.2.4. Helicase-Dependent Amplification (HDA)
4.2.5. Rolling Circle Amplification (RCA)
4.3. Digital Amplification
5. Detection
6. Novel Microfluidic Actuators
6.1. Paper-Based Microfluidics
6.2. Centrifugal-Based Microfluidics
6.3. Digital Microfluidics
7. Multiplex Integrated Devices
8. Commercial Microfluidic Diagnosis
9. Summary and Conclusions
10. Future Outlook
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Basha, I.H.K.; Ho, E.T.W.; Yousuff, C.M.; Hamid, N.H.B. Towards Multiplex Molecular Diagnosis—A Review of Microfluidic Genomics Technologies. Micromachines 2017, 8, 266. https://doi.org/10.3390/mi8090266
Basha IHK, Ho ETW, Yousuff CM, Hamid NHB. Towards Multiplex Molecular Diagnosis—A Review of Microfluidic Genomics Technologies. Micromachines. 2017; 8(9):266. https://doi.org/10.3390/mi8090266
Chicago/Turabian StyleBasha, Ismail Hussain Kamal, Eric Tatt Wei Ho, Caffiyar Mohamed Yousuff, and Nor Hisham Bin Hamid. 2017. "Towards Multiplex Molecular Diagnosis—A Review of Microfluidic Genomics Technologies" Micromachines 8, no. 9: 266. https://doi.org/10.3390/mi8090266